Name: Assoc. Prof. Dr. Mayuree FuangthongOffice address: Laboratory of Biotechnology54 Kamphaeng Phet 6 Talat Bang Khen, Lak Si, Bangkok 10210, ThailandTel: +66 2553 8555(ext. 3251), +66 2554 1900E-mail: [email protected] ScientistResearch Experience (Specialties) Gene regulation in pathogenic bacteria Education B.Sc. (Medical technology) Mahidol University, Bangkok M.Sc. (Biotechnology) Mahidol University, Bangkok Ph.D. (Microbiology) Cornell University, USA PublicationsMethylation at position 32 of tRNA catalyzed by TrmJ alters oxidative stress response in Pseudomonas aeruginosa. Jaroensuk J, Atichartpongkul S, Chionh YH, Wong YH, Liew CW, McBee ME, Thongdee N, Prestwich EG, DeMott MS, Mongkolsuk S, Dedon PC, Lescar J, Fuangthong M.Nucleic Acids Res. 2016 Dec 15;44(22):10834-10848. Epub 2016 Sep 28. Regulation of organic hydroperoxide stress response by two OhrR homologs in Pseudomonas aeruginosa.Atichartpongkul S, Vattanaviboon P, Wisitkamol R, Jaroensuk J, Mongkolsuk S, Fuangthong M.PLoS One. 2016 Aug 25;11(8):e0161982. doi: 10.1371/journal.pone.0161982. eCollection 2016. IscR plays a role in oxidative stress resistance and pathogenicity of a plant pathogen,Xanthomonas campestris.Fuangthong M, Jittawuttipoka T, Wisitkamol R, Romsang A, Duang-nkern J, Vattanaviboon P, Mongkolsuk S.Microbiol Res. 2015 Jan;170:139-46. doi: 10.1016/j.micres.2014.08.004. Epub 2014 Aug 22. Derepression of the Bacillus subtilis PerR peroxide stress response leads to iron deficiency.Faulkner MJ, Ma Z, Fuangthong M, Helmann JD.J Bacteriol. 2012 Mar;194(5):1226-35. doi: 10.1128/JB.06566-11. Epub 2011 Dec 22. Exposure of Acinetobacter baylyi ADP1 to the biocide chlorhexidine leads to acquired resistance to the biocide itself...

IACUC form 500 to 505 LAU-IACUC Form 500 PROTOCOL COVER SHEET LAU-IACUC Form 501 IACUC memorandum to PI (protocol review) LAU-IACUC Form 502 Documentary Proof of Ethical Clearance LAU-IACUC Form 503 IACUC memorandum to PI (Notification of Protocol Approval) LAU-IACUC Form 504 Protocol Amendment LAU-IACUC Form 505 Annual Review of Animal Protocol

Previous Next The objective of the Occupational Health and Safety (OHS) Program is to outline the process for identifying health and safety concerns, towards the reduction of the occurrence of occupational incidents, injuries, and illnesses, and to promote employee health and well-being. All LAC personnel, animal researchers, visitors and maintenance personnel who will conduct any kinds of working in the animal facility must be enrolled in the OHS program. They are requested to submit a completed OHS survey form to the OHS office. The OHS Committee will then prepare a risk management worksheet customized to the personnel based on the information submitted. Some personnel may be required to receive certain vaccines as protection against infectious disease or suggested to use specialized PPE if allergy or any health problem arises. The OHS Committee reviews and consults all animal use protocols for identification of potential chemical and biological hazards parallel to review by the IACUC. The committee reviews and discusses safety matters involving the use of chemical, biohazardous and radiological agents or materials in animal protocols to ensure the investigator has appropriately planned to reduce or eliminate the exposure to potentially hazardous agents. The Occupational Health and Safety Committee conducts safety inspections of the LAC, including practices and techniques, safety equipment, facility engineering, facility activities and agents used. , usually at the...

              Previous Next All personnel working with animals are trained prior to commencing work 1. Ethics and welfare in animal experimentation and protocol writing for investigators who will prepare the animal use protocol 2. Animal training procedures as requested depending on the specific animal protocol 3. Occupational health and safety Routine prophylaxis and screening Personnel Protective Equipment General Biosafety and Blood-borne Pathogens Chemical safety General Laboratory Safety and Lab Waste Management Safety shower and eyewash training Fire Prevention-Emergency Preparedness and Rescue Plan 4. LAC-SOP training related to individual task

              Previous Next Animal Environment The temperature is regulated at 22±1oC, with a relative humidity (RH) of 55±10% and air exchange rate is set not to fall below 15 air changes/hr. throughout each 24-hour period. The temperature RH and air exchange rate in each room are monitored and recorded in real time by a computer monitoring system. Sound level and light intensity is not exceed 85 dB, 325 lux respectively in animal housing area. Ammonia levels should not exceed 25 ppm in any type of animal facility. Animal Supplies Supplies must have certification as the standard values. Each lot of supplies are sampled and tested for microbial contamination, heavy metal or others that researcher or veterinarian requested by the outsource certified laboratory. Micro- and Macro-Environments Cages and lids, filtered top cages, ventilated cages, water bottles, rack/shelves, laminar flow racks, safety cabinets and other animal equipment are routine sanitized in scheduled frequency. The effectiveness of sanitization procedures is monitored (e.g., water temperature monitoring, sterility test, microbiological monitoring, visual inspection and other indicators) Animal rooms sanitation is monitored using air sampler routinely

CRI is committed to the humane care and use of all research animals at the institute. It is the responsibility of each and every employee to report all observed cases or evidence of animal abuse or negligence immediately. The mechanisms for reporting concerns are posted in public areas, e.g. the daily sign in area for employees. M floor of LAC Building Entrance 2nd floor of LAC Building Entrance LAC Office at M floor of Biomedical Science Building G floor of Biomedical Science Building Entrance E-mail: [email protected] or Contact The methods for reporting and investigating animal welfare concerns are as follow: Reports can be made without fear of retaliation. The reporting individual can submit anonymously via Written Reports of animal welfare concerns (include a description of the animal health concern, the location and species involved, names of any parties involved or witnessing the activity in question, and protocol numbers or other pertinent information) can be e-mailed or delivered directly to the following persons from the post Verbal Reports of animal welfare concerns can be given to the same individuals listed from the post, and should include the descriptions listed as report by writing The IACUC chair will bring the issue to the IACUC. The results of all completed investigations will be reviewed by the IACUC All...

The IACUC must review all proposed animal use protocols before any animal research can be performed within the CRI research and animal facilities. In reviewing the protocol, the IACUC will consider whether the protocol contains all required management and regulatory elements as defined by the Animal Use Policy and Laboratory Animal Program Description. The IACUC will determine if the proposal contains the following information: a justification of why the treatment/condition is scientifically necessary; a statement demonstrating that the PI performed a thorough search of alternatives to the potentially painful procedures; and standards and practices for pain management, adjuvant use, humane end-point determination, etc. At the same time, the OHS Committee will review and discuss safety matters involving the use of chemical, biohazardous and/or radiological agents or materials in animal protocols to ensure the investigator has appropriately planned to reduce or eliminate the exposure to potentially hazardous agents. After determination by the IACUC that the animal use protocol is complete, the protocol is recommended to the IO (or Vice-President for Research and Academic Affairs in the case that the IO is part of the research team on the protocol) for final approval. After official approval, the IACUC Chair will inform the PI that the study may begin. Links 1. LAC-IACUC Form 500 PROTOCOL COVER SHEET2. LAC-IACUC Form 501 IACUC memorandum to...

Proposed modifications to an approved protocol must be reviewed by the IACUC, and receive final approval from the IO (or Vice-president for Research and Academic Affairs in the case that the IO is part of the research team on the protocol) prior to start of the requested study. Modifications to animal protocols may be classified as minor or major. Minor modifications may include changing doses or routes of administration of a drug, and recording or measuring additional variables in the whole animals that are no more invasive than procedures currently approved under the protocol, and that do not increase the pain category of the protocol. Major modifications are those that require a change in the scientific direction of a protocol, e.g. requiring a greater than 10% increase in the number of test animals, changes in the species of the animals approved on the original protocol, changes in pain severity rating, increases in biohazard level on the protocol, and the addition of multiple minor modifications, or a change in the PI. A new PI for a previously approved and active protocol must submit a training statement with his/her request and a signed assurance statement. The process for reviewing/approving protocol amendments is the same as for reviewing a new protocol. Links 1. LAC-IACUC Form 500 PROTOCOL COVER SHEET2....

Annual review and approval by the CRI-IACUC is required for each protocol to remain active through the approved period (3 years). Annual reviews are generally due at the end of each year and cover the previous fiscal year. Amendments should be incorporated into the body of the text and required literature searches must be updated by the Principle Investigator (PI) via an Annual Review of Animal Protocol (IACUC form 505) submitted to the IACUC Administrator for IACUC review and approval. If animal work is to continue on a protocol after the three-year period, the protocol is required to be rewritten completely and all information updated prior to a full IACUC review. In addition, post approval monitoring (PAM) is randomly conducted by means of: a) Additional reviews conducted as appropriate if and deviations or violations are detected/reported. b) Routine observation of animals by animal care personnel. c) Veterinary care or observation by the Attending Veterinarian.

Previous Next Animal research conducted at CRI includes the search for new anti-malarial drugs, and factors affecting susceptibility to malarial infection; studies on animal models of various types of cancers; toxicity of metals, pesticides and air pollutants; acute and chronic toxicity of chemicals found in natural sources; and the health effects of climate change. The major users of the animal facility are researchers from laboratories of Environmental Toxicology, Pharmacology, Immunology, as well as Translational Research Center.

Previous Next 2 Full-time veterinarians (1 Attending Veterinarian and 1 Clinical Veterinarian) has direct responsibility for ensuring adequate veterinary care and is also responsible for ensuring adequate resources to care for animals; proper methods to prevent diseases; communication regarding animal health issues; advising technical and professional staff on proper animal care and use; ensuring proper care and storage of equipment and supplies; and all other aspects of the veterinary care program. Also, the duties engage in protocol support discussion with investigators and coordinates with the veterinary technician. The veterinary technician and animal caretakers are available during the workday from 9 am to 5 pm. They observe the animals during feeding and/or treatment/protocol-related activities. The animals are observed at least once daily also weekend and holiday. After hours observation or treatment is done on an “as needed” basis, when there is a scheduled protocol or emergency medical attention is required.

  Guideline     Guide for the care and use of Laboratory Animals (EN) Guide for the care and use of Laboratory Animals (TH) Occupational Health the Care and Use AVMA Guidelines For Euthanasia   Document   จรรยาบรรณการดำเนินการต่อสัตว์เพื่องานทางวิทยาศาสตร์ พศ2559 Ethical Principles and Guidelines for the Use of Animals พระราชบัญญัติสัตว์เพื่องานทางวิทยาศาสตร์ พ.ศ. 2558 (ฉบับภาษาไทย) พระราชบัญญัติสัตว์เพื่องานทางวิทยาศาสตร์ พ.ศ. 2558 (ฉบับภาษาอังกฤษ) จรรยาบรรณนักวิจัย Researcher’s Code of Ethics ข้อกําหนดจรรยาบรรณการดําเนินการต่อสัตว์เพื่องานทางวิทยาศาสตร์ ข้อกําหนดจรรยาบรรณการดําเนินการต่อสัตว์เพื่องานทางวิทยาศาสตร์ (ฉบับที่2) พ.ศ.2560 Link 1.The Association for Assessment and Accreditation of Laboratory Animal Care (AAALAC) 2.Thai Association for Laboratory Animal Science (TALAS) 3.สถาบันพัฒนาการดำเนินการต่อสัตว์เพื่องานทางวิทยาศาสตร์ (สพสว.) 4.American Association for Laboratory Animal Science learning library (AALAS learning library)