Name: Dr. Ormrat Kampeerawipkakorn Office address: Environmental Toxicology Laboratory, Biomedical Building 54 Kamphaeng Phet 6 Talat Bang Khen, Lak Si, Bangkok 10210, Thailand Tel: +66 2553 8555 (ext. 3202) E-mail: [email protected] Research Scientist Education Bachelor of Nursing Science (First Honor) Faculty of Nursing, Mahidol University, Bangkok, Thailand Ph.D. in Toxicology Faculty of Science, Mahidol University, Bangkok, Thailand Research Interest Principle research interests lie in the field of genotoxic pollutants such as air, water and soil pollutants. The studies mainly show the association of genotoxic pollutants and human health effects. Publications Exposure to genotoxins present in ambient air in Bangkok, Thailand–particle associated polycyclic aromatic hydrocarbons and biomarkers. Ruchirawa M, Mahidol C, Tangjarukij C, Pui-ock S, Jensen O, Kampeerawipakorn O, Tuntaviroon J, Aramphongphan A, Autrup H Sci Total Environ287p121-32(2002 Mar 15)  

Name: Dr. Sittisak Pui-ock Office address: Research Laboratory of Environmental Toxicology, Chulabhorn Research Institute, 54 Kamphaeng Phet 6 Talat Bang Khen, Lak Si, Bangkok 10210, Thailand Tel: +662-553-8555 ext. 3103 (office) Fax: +662-553-8565 Positions: Research Scientist, Research Laboratory of Environmental Toxicology, Chulabhorn Research Institute E-mail: [email protected] Website: https://liquid-biopsy.my.canva.site/liquid-biopsy Research Interests Detection and isolation of circulating tumour cells from blood by different methods DEP-FFF technique or filtration technique available in commercial kits for circulating tumor cells isolation Education and Degrees 1993 Bachelor of Science (Food Science and Technology), ChiangMai University (CMU), Thailand 1996 Master of Applied Science (Food Science and Nutrition), University of Western Sydney, Hawkesbury, Australia 2008 Doctor of Technical Science in Environmental Toxicology, Technology and Management (ETTM), Asian Institute of Technology (AIT), Thailand Postdoctoral Fellowship Visiting postdoctoral fellow to Department of Molecular Pathology (Unit 951), MD Anderson USA. During October to December, 2009. Visiting purpose to obtain in-depth training and experience in the isolation of viable circulating tumour cells by continuous-flow dielectrophoretic field-flow fractionation (CF-DEP-FFF) Professional Societies Member: Thai Society of Toxicology, 2012-present ORCID iD: https://orcid.org/0000-0002-4309-8242 Publications A. Research Articles in International Journals Pui-ock S, Ruchirawat M, Gascoyne P. Dielectrophoretic field-flow fractionation system for detection of aquatic toxicants. Anal Chem 80(20):7727-34, 10/2008. e-Pub 9/2008. PMCID: PMC2726257 Ruchirawat M, Mahidol C, Tangjarukij C, Pui-ock S, Jensen O, Kampeerawipakorn O, Tuntaviroon J, Aramphongphan A,...

Name: Dr. Daam Settachan Office address: Environmental Toxicology Laboratory, Biomedical Building 54 Kamphaeng Phet 6 Talat Bang Khen, Lak Si, Bangkok 10210, Thailand Tel: +66 2553 8555 (ext. 3105) E-mail: [email protected] Position: Research Scientist Research Interest – Development and refinement of whole-effluent toxicity tesing with local indicator species as a monitoring tool for wastewater effluent quality – Rodent models for inhalation toxicity testing to determine health effects from exposure to environmental toxicants Education B.S. (Biology) – Mahidol University M.S. (Environmental Toxicology) – Clemson University (USA) Ph.D. (Environmental Toxicology) – Texas Tech University (USA) Publications Exposure to benzene in various susceptible populations: co-exposures to 1,3-butadiene and PAHs and implications for carcinogenic risk. Ruchirawat M, Navasumrit P, Settachan D Chem Biol Interact184p67-76(2010 Mar 19) PCBs contamination in seafood species at the Eastern Coast of Thailand. Jaikanlaya C, Settachan D, Denison MS, Ruchirawat M, van den Berg M Chemosphere76p239-49(2009 Jun) Assessment of potential cancer risk in children exposed to urban air pollution in Bangkok, Thailand. Ruchirawat M, Settachan D, Navasumrit P, Tuntawiroon J, Autrup H Toxicol Lett168p200-9(2007 Feb 5) Environmental impacts on children’s health in Southeast Asia: genotoxic compounds in urban air. Ruchirawat M, Navasumrit P, Settachan D, Autrup H Ann N Y Acad Sci1076p678-90(2006 Sep) Measurement of genotoxic air pollutant exposures in street vendors and school children in and near Bangkok. Ruchirawat...

Name: Assoc. Prof. Dr. Panida Navasumrit Office address: Environmental Toxicology Laboratory, Biomedical Building 54 Kamphaeng Phet 6 Talat Bang Khen, Lak Si, Bangkok 10210, Thailand Tel: +66 2553 8555 (ext. 8672) E-mail:

Snake venoms are produced by snakes for digestion and to rapidly immobilize their prey. Venoms contain various hydrolytic enzymes and toxins e.g., neurotoxins and cytotoxins. Neurotoxic venoms are mainly produced by elapid snakes (cobra, krait, mamba) and sea snakes. These toxins cause neuromuscular blockage, leading to respiratory failure and death. Postsynaptic neurotoxins (PSNTs) from different elapids, although chemically and pharmacologically similar, are immunochemically distinct, so antivenom (AV) against venom from one elapid usually fails to neutralize venom of another elapid, even from the same genus. Thus, each country or region needs to produce its own AV, which is not economically or technically always possible. The lack of effective AVs has resulted in numerous deaths of snakebite victims. Our aim is to produce a pan–specific antivenom against venoms of Southeast Asian elapid snakes. Previously we have established an immunization protocol (WHO Guidelines, 2010), which is highly effective in producing high titer and high affinity antibody against PSNTs of various snakes. Potent polyspecific antivenom has been produced against 3 elapids from Thailand with ED50s comparable to the corresponding monospecific antivenoms. Using this immunization protocol with selected neurotoxins from various elapids, we are studying the preparation of a pan-specific antivenom against elapids of SE Asian countries. If successful, this antivenom could be produced for wide distribution and, with economy of scale, should...

Recently, there is an urgent need for rapid diagnostic tests for new infectious agents in man and animals. Conventionally, these infectious agents are identified either by culture or by using molecular biology techniques, e.g. PCR. These processes are costly and usually take several hours or even days. We have produced luminol encapsulated biotinylated liposome and used it as a generic signal generator, capable of conjugation to biotinylated biomolecules, such as specific microbial oligonucleotides, antibody and antigen. The system has been used to detect specific oligonucleotide hybridization and specific antigen-antibody interaction, and the results have been published.

Human pythiosis is an emerging and life-threatening infectious disease caused by the fungus-like organism Pythium insidiosum. It is endemic in Thailand where about 80% of the cases are reported. The disease has high rate of morbidity and mortality, due to lack of early diagnosis and effective treatment. We have developed a convenient and reliable immunochromatographic test (ICT) for rapid detection of human anti-P.insidiosum IgG, for use in early diagnosis and monitoring of pythiosis patients, resulting in saving of limbs and lives. The test kit has been mass produced for free distribution to 6 hospitals with medical schools throughout the country. This research has been published and a patent application has been submitted. In collaboration with researchers from the Department of Pathology, Ramathibodi Hospital, the ICT has been used to study the seroprevalence of P.insidiosum infection in high-risk hematological patients, and a multi-host ICT for the detection of P.insidiosum infection in human, horse and dog has been developed.  

Development of immunochromatographic tests for simple diagnosis of human gnathostomiasis caused by Gnathostoma spinigerumGnathostomiasis is a parasitic infection caused by the third stage larvae of the helminthes Gnathostoma spp., and is found mostly in tropical and subtropical regions. It is a food-borne zoonosis and is endemic in areas where people eat raw freshwater fish or shellfish, especially Thailand and other parts of Southeast Asia, Japan, and increasingly Latin America, particularly Mexico. The common clinical features include an intermittent cutaneous or subcutaneous migratory swelling that may persist for many years. In some cases, the parasite can enter other tissues such as the liver, and the eye, resulting in vision loss or blindness, and the nerves, spinal cord, or brain, resulting in nerve pain, paralysis, coma and death.In this project, we propose to study to produce a recombinant protein from a specific antigen and/or synthesize a specific peptide which will be used as a specific antigen of G. spinigerum and to develop a highly specific and sensitive immunochromatographic test for gnathostomiasis.  

Name: Mrs. Supornrat Pulleium Office address: Research Laboratory of Chemical Carcinogenesis, Chulabhorn Research Institute, 54 Kamphaeng Phet 6 Talat Bang Khen, Lak Si, Bangkok 10210, Thailand Tel: +66 25530 8555 (ext. 3130) E–mail:[email protected] Position: Research Scientist Education and Degreees B.Sc. (Medical technology), Mahidol University, 1988–1991 M.Sc. (Microbiology), Mahidol University, 1992–1994 Work Activities Research Scientist,Laboratory of Chemical Carcinogenesis, Chulabhorn Research Institute, 1995–present

Name: Miss Somkid Sitthimonchai Office address: Research Laboratory of Chemical Carcinogenesis, Chulabhorn Research Institute, 54 Kamphaeng Phet 6 Talat Bang Khen, Lak Si, Bangkok 10210, Thailand Tel: +662–5538555 (ext. 3127-8) (office) Fax:+662–553–8572 Positions:Research Scientist, Research Laboratory of Chemical Carcinogenesis, Chulabhorn Research Institute E-mail:[email protected] Research Interests Biochemistry Education and Degrees B.Sc. (Chemistry), Chiang Mai University, Thailand, 1988-1992 M.Sc. (Biochemistry), Mahidol University, Thailand, 1997-2001 Academic Positions Research Assistant, Chemical Carcinogenesis laboratory, Chulabhorn Research Institute, 1994-1997 Lab Training for Cancer Chemoprevention Screening at German Cancer Research Center (DKFZ), Heidelberg, Germany, 2001-2002 Research Scientist, Chemical Carcinogenesis laboratory, Chulabhorn Research Institute, 2001-present Publications Bhinija, K., Huehne, P. S., Mongkolsuk, S., Sitthimonchai, S., & Satayavivad, J. (2022). A short-chain dehydrogenase/reductase (SDR) detection for the Isoflavone reductase gene in Bulbophyllum and other orchids. South African Journal of Botany, 144, 295-304. doi:https://doi.org/10.1016/j.sajb.2021.08.034 Kheawchaum, S., Mahidol, C., Thongnest, S., Boonsombat, J., Batsomboon, P., Sitthimonchai, S., . . . Prawat, H. (2022). Ent-abietane diterpenoid lactone glycosides and a phenolic glycoside from Phlogacanthus pulcherrimus T. Anderson with cytotoxic and cancer chemopreventive activities. Phytochemistry, 201, 113261. doi:10.1016/j.phytochem.2022.113261 Prachayasittikul, V., Pingaew, R., Worachartcheewan, A., Sitthimonchai, S., Nantasenamat, C., Prachayasittikul, S., . . . Prachayasittikul, V. (2017). Aromatase inhibitory activity of 1,4-naphthoquinone derivatives and QSAR study. EXCLI J, 16, 714-726. doi:10.17179/excli2017-309 Phakeovilay, C., Disadee, W., Sahakitpichan, P., Sitthimonchai, S., Kittakoop, P., Ruchirawat, S., &...

Name: Mister Nirat Pholphana Office address: Research Laboratory of Chemical Carcinogenesis, Chulabhorn Research Institute, 54 Kamphaeng Phet 6 Talat Bang Khen, Lak Si, Bangkok 10210, Thailand Tel: +66 2553 8555 (ext. 3131) Fax:+662-553-8572 E-mail:[email protected] Positions:Research Scientist, Research Laboratory of Chemical Carcinogenesis, Chulabhorn Research Institute Education and Degrees B.Sc. (Medical Technology), Chulalongkorn University, 1991 M.Sc. (Microbiology), Mahidol University, 1995

Name: Dr. Pattama Singhirunnusorn Office address: Research Laboratory of Chemical Carcinogenesis, Chulabhorn Research Institute, 54 Kamphaeng Phet 6 Talat Bang Khen, Lak Si, Bangkok 10210, Thailand Tel: +662–5538555 ext. 3135 (office), +6687–5915431 (mobile) Fax:+662–5538572 Positions:Research Scientist, Research Laboratory of Chemical Carcinogenesis, Chulabhorn Research Institute E–mail:[email protected] Website:– Research Interests Signal transduction Cell and Molecular Biology Education and Degrees: B.Sc. (Chemistry), Suan Dusit Rajabhat University, Bangkok, Thailand, 1994–1997 M.Sc. (Pharmaceutical Chemistry), Mahidol University, Bangkok, Thailand, 1999–2002 Ph.D. (Pharmaceutical Science), University of Toyama, Toyama, Japan, 2002–2005 Academic Positions Researcher, Department of Pharmacognosy, Faculty of pharmacy, Mahidol University, Thailand, 1998 Postdoctoral Fellow, Division of Pathogenic Biochemistry, Department of Bioscience, Institute of Natural Medicine, University of Toyama, Japan, 2006–2008 Researcher, Chemical Carcinogenesis laboratory, Chulabhorn Research Institute, 2008–present Academic Activities Local Activities Project proposal committee (2015–2016), Thesis defend committee (2016–2017), Anti–Aging and Regenerative Medicine Faculty, Dhurakij Pundit University Fellowships Postdoctoral Fellow, Division of Pathogenic Biochemistry, Department of Bioscience, Institute of Natural Medicine, University of Toyama, Japan, 2006–2008 International Awards & Honours Toyama New Industry Organization Grants, Japan, 2006–2007 Monbukagakusho Scholarship, Japan, 2002–2005 Local Awards & Honours Poster award at The 10th National Cancer Conference, Bangkok, Thailand, 2009 ORCID iD: https://orcid.org/0000–0002–8085–5417 Publications A. Research Articles in International Journals Singhirunnusorn, P., Moolmuang, B., Lirdprapamongkol, K., & Ruchirawat, M. (2018). Arsenite exposure potentiates apoptosis–inducing effects of tumor necrosis...