Name: Ms. Kisana Bhinija Office address: Laboratory of Biotechnology 54 Kamphaeng Phet 6 Talat Bang Khen, Lak Si, Bangkok 10210, Thailand Tel: +66 25530 8555 (ext. 3033, 3249) E-mail: Position: Research Scientist I Research Experience (Specialties) Plant tissue culture, Plant cytogenetics, Plant proteomic Education 1992-1995 B. Sc. (Agriculture) Khonkean University 1997-2002 M. Sc. (Genentics) Kasetsart University Publications Patipipat, P., Bhinija, K., Wongkhamprai, B., Hunhne, P. S. 2014. Molecular cloning of arsenate reductase and phytochelatin synthase genes related to arsenic response in Oryza sativa L. ssp. indica cv. Khao Dawk Mali 105. Thai J. Genet. 7(1) : 41–50. Mohamad, Z. A., Chokchaichamnankit, D., Bhinija, K., Paricharttanakul, N. M., Svasti, J., Huehne, P. S., Srisomsap, C. 2013. Proteome level changes in the root of Brassica alboglabra induced by alachlor herbicide. African Journal of Biotechnology. 12(20) : 2840-2849. Huehne, P. S., Bhinija, K. 2012. Application of cryoprotectants to improve low temperature storage survival of orchid seeds. Scientia Horticulturae. 135(1) : 186-193. Mohamad, Z. A., Chokchaichamnankit, D., Bhinija, K., Paricharttanakul, N. M., Svasti, J., Huehne, P. S., Srisomsap, C. 2011. Proteomic analysis of Alboglabra leaves during growth. Jiomic 1(1): 102-107. Lertwiriyawong, B., Bhinija K., Huehne., P.S. 2010. Sequence Analysis of the 16SrRNA-rps12 Inverted Repeat Region in Chloroplast DNA of Dendrobium orchids. Kasetsart J. Nat. Sci. 45 : 45-472. Teerarak, M., Bhinija, K.,Thitavasanta, S., Laosinwattana,...

Name: Dr. Panu Ruangjan Office address: Laboratory of Biotechnology 54 Kamphaeng Phet 6 Talat Bang Khen, Lak Si, Bangkok 10210, Thailand Tel: +66 25530 8555 (ext. 3033) E-mail: [email protected] Research Scientist Research Experience (Specialties) Plant Breeding, Plant Molecular Biology Education 1981-1985 B. Sc (Horticulture) Kasetsart University 1986-1990 M. Sc (Horticulture) Kasetsart University 2001-2005 Ph. D (Molecular Genetic and Genetic Engineering Mahidol University Awards 2001-2005 Scholarship from the Civil Service Commission Training Workshop 2003 Workshop on Genetic Diversity Analysis Using DNA Marker during May15 – 19 at Department of Genetic, Kasetsart University 2003 Workshop on Linkage Map Construction and Quantitative Trait Loci (QTL) Analysis during May 22 – 26 at Rice Gene Discovery Unit, National Center for Genetic Engineering and Biotechnology 2007 Workshop on International Workshop on Ornamental Plants during January 8 – 11 at Lotus Hotel Pang Suan Kaew 2010 Workshop on Biotechnology Methods for Gynogenesis of Cucumber and Androgenesis of Pepper during March 17 – 19 at National Center for Genetic Engineering and Biotechnology, Kamphaeng Saen Unit Publications 1997 D. Evenor, H. Lilien-Kipnis, A.A. Watad and P. Raungjan, 1997. Micropropagation of ornamental Allium aflatunense and ampeloprasum. ISHS Acta Horticulturae 447, III International Symposium on In Vitro Culture and Horticulturae Breeding, Jerusalem, Israel, p. 135-138 (Poster Presentation) 2002 Ruangjan, P., M. Juricek, S. Panyim. and S. Kerdbandit, 2002. Analysis of...

Name: Mister Weerachai Tanboon Office address: Research Laboratory of Biotechnology, Chulabhorn Research Institute, 54 Kamphaeng Phet 6 Talat Bang Khen, Lak Si, Bangkok 10210, Thailand Tel: +662-5538555 (ext. 8556) (office) Positions: Research Scientist, Research Laboratory of Biotechnology, Chulabhorn Research Institute E-mail: [email protected] Research Interests Molecular microbiology Education and Degrees M.B.A. (General Management), Chandrakasem Rajabhat University, Thailand, 2008 B.Sc. (Microbiology), King Mongkut’s University of Technology, Thailand, 2001 Publications Tanboon, W., Chuchue, T., Vattanaviboon, P., & Mongkolsuk, S. (2009). Inactivation of thioredoxin-like gene alters oxidative stress resistance and reduces cytochrome c oxidase activity in Agrobacterium tumefaciens. FEMS Microbiology Letters, 295(1), 110-116. doi:10.1111/j.1574-6968.2009.01591.x Vattanaviboon, P., Tanboon, W., & Mongkolsuk, S. (2007). Physiological and expression analyses of Agrobacterium tumefaciens trxA, encoding thioredoxin. Journal of Bacteriology, 189(17), 6477-6481. doi:10.1128/JB.00623-07 Chuchue, T., Tanboon, W., Prapagdee, B., Dubbs, J. M., Vattanaviboon, P., & Mongkolsuk, S. (2006). ohrR and ohr are the primary sensor/regulator and protective genes against organic hydroperoxide stress in Agrobacterium tumefaciens. Journal of Bacteriology, 188(3), 842-851. doi:10.1128/JB.188.3.842-851.2006

Name: Miss Jintana Duang-nkern Office address: Laboratory of Biotechnology 54 Kamphaeng Phet 6 Talat Bang Khen, Lak Si, Bangkok 10210, Thailand Tel: +66 25530 8555 (ext. 3261) E-mail: Position: Research Scientist I Research Experience (Specialties) Molecular Genetics and Molecular Biochemistry in Microbiology 2011-Present Chulabhorn Research Institute, Laboratory of Biotechnology in Projects: -Molecular characterization of the repair systems that protect pathogenic bacteria from toxicants-induced DNA damages. -Analysis of multi-drug and stress resistance mechanisms in soil and pathogenic bacteria. -Identification of novel genes that involve in bacterial pathogenesis 2008–2010 Mahidol University, Faculty of Science and Department of Biotechnology Research Project: Characterization of the nfuA, encoding an iron sulfur scaffold protein in Pseudomonas aeruginosa 2006 –2007 Kasertsart University, Faculty of Science and Department of Genetics Research Project: Indentification Hybrid Mushroom of Lentinula edodes, Lentinus squarrosulus and Lentinus polychrous Using AFLP Techinque 2006 National Center for Genetic Engineering and Biotechnology (BIOTEC), Protein-Ligand Engineering and Molecular Biology Laboratory Topic: Cloning of pvPPPK-DHPS into pPICZA for protein expression in yeast Pichia sp. and Expression and purification of peptides from Pvpppk-dhps for generation of antibody production. Education 2008-2010 M.Sc. in Biotechnology Mahidol University 2003-2006 B.Sc. in Biology (Genetics) Kasetsart University Awards 2006 Second Class Honor from Kasetsart University 2005 Certificate of Accomplishment for The Molecular Biology Training at National Center for Genetic Engineering and Biotechnology (BIOTEC), National...

Name: Ms. Wirongrong Whangsuk Office address: Laboratory of Biotechnology 54 Kamphaeng Phet 6 Talat Bang Khen, Lak Si, Bangkok 10210, Thailand Tel: +66 25530 8555 (ext. 3258) E-mail: [email protected] Research Scientist Research Experience (Specialties) Molecular microbiology Education 1988-1991 B. Sc. (Kasetsert) Kasetsart University 1998-2000 M. Sc. (Biotechnology) Mahidol University Publications – Sallabhan R, Kerdwong J, Dubbs JM, Somsongkul K, Whangsuk W, Piewtongon P, Mongkolsuk S, Loprasert S. The hdhA Gene Encodes a Haloacid Dehalogenase that is Regulated by the LysR-Type Regulator, HdhR, in Sinorhizobium meliloti. Mol Biotechnol. 2012 May 26. – Whangsuk W, Sungkeeree P, Thiengmag S, Kerdwong J, Sallabhan R, Mongkolsuk S, Loprasert S. Gene Cloning And Characterization of a Novel Highly Organic Solvent Tolerant Lipase from Proteus sp. SW1 and its Application for Biodiesel Production. Mol Biotechnol. 2012 Feb 28. – Whangsuk W, Dubbs JM, Sallabhan R, Somsongkul K, Mongkolsuk S, Loprasert S. ChpR is a chlorpyrifos-responsive transcription regulator in Sinorhizobium meliloti. J Mol Microbiol Biotechnol. 2010;18(3):141-7. Epub 2010 Apr 13. – Loprasert S, Whangsuk W, Dubbs JM, Sallabhan R, Somsongkul K, Mongkolsuk S. HpdR is a transcriptional activator of Sinorhizobium meliloti hpdA, which encodes a herbicide-targeted 4-hydroxyphenylpyruvate dioxygenase. J Bacteriol. 2007 May;189(9) : 3660-4. Epub 2007 Mar 2. – Whangsuk W, Mongkolsuk S. Analysis of mutations that alter H2O2 sensing and transcription regulation properties of a global...

Name: Mr. Ratiboot Sallabhan Office address: Laboratory of Biotechnology 54 Kamphaeng Phet 6 Talat Bang Khen, Lak Si, Bangkok 10210, Thailand Tel: +66 25530 8555 (ext. 3257) E-mail: Research Scientist Research Experience (Specialties) Molecular microbiology Education 1999 M.Sc. (Biotechnology) Mahidol University, Thailand 1992 B.Sc. (Biology), Ramkhamhang University, Thailand Publications 1) The hdhA gene encodes a haloacid dehalogenase that is regulated by the LysR-type regulator, HdhR, in Sinorhizobium meliloti. Sallabhan R, Kerdwong J, Dubbs JM, Somsongkul K, Whangsuk W, Piewtongon P, Mongkolsuk S, Loprasert S. Mol Biotechnol. 2013 Jun;54(2):148-57. 2) Gene cloning and characterization of a novel highly organic solvent tolerant lipase from Proteus sp. SW1 and its application for biodiesel production. Whangsuk W, Sungkeeree P, Thiengmag S, Kerdwong J, Sallabhan R, Mongkolsuk S, Loprasert S. Mol Biotechnol. 2013 Jan;53(1):55-62. 3) Bacillus subtilis SSE4 produces subtulene A, a new lipopeptide antibiotic possessing an unusual C15 unsaturated beta-amino acid. Thasana N, Prapagdee B, Rangkadilok N, Sallabhan R, Aye SL, Ruchirawat S, Loprasert S. FEBS Lett. 2010 Jul 16;584(14):3209-14. doi: 10.1016/j.febslet.2010.06.005. Epub 2010 Jun 10. 4) ChpR is a chlorpyrifos-responsive transcription regulator in Sinorhizobium meliloti. Whangsuk W, Dubbs JM, Sallabhan R, Somsongkul K, Mongkolsuk S, Loprasert S. J Mol Microbiol Biotechnol. 2010;18(3):141-7. 5) Mutations of ferric uptake regulator (fur) impair iron homeostasis, growth, oxidative stress survival, and virulence of Xanthomonas campestris pv. campestris....

Name: Assoc. Dr. Oranart Matangkasombut Office address: Laboratory of Biotechnology54 Kamphaeng Phet 6 Talat Bang Khen, Lak Si, Bangkok 10210, ThailandTel: +66 25530 8555 (ext. 3251)E-mail: [email protected] Research Scientist Research Experience (Specialties) Molecular biology Education 1988-1994 DDS, Chulalongkorn University 1996-2002 PhD (Biological Sciences in Dental Medicine), Harvard University 2002-2004 Postdoctoral fellowship (with Prof. Gerald R.Fink), Whitehead Institute for Biomedical Research, MA, USA Awards 1995 Anandamahidol scholarship in Dentistry 2002 Harvard School of Dental Medicine Dean’s scholar award Publications– Cefai D., Schneider H., Matangkasombut O., Kang H., Brody J., Rudd C.E. (1998) CD28 receptor endocytosis is targeted by mutations that disrupt phosphatidylinositol 3-kinase binding and costimulation. J Immunol,160(5):2223-30 – Matangkasombut O., Buratowski R.M., Swilling N., Buratowski S. (2000) Bromodomain factor1 corresponds to a missing piece of yeast TFIID. Genes.Dev. 14(8):951-6 – Matangkasombut O., and Buratowski S. (2003) Different sensitivities of bromodomain factors 1 and 2 to histone H4 acetylation. Mol. Cell 11(2):353-63 – Matangkasombut O., Auty R., Buratowski S. (2004) Structure and function of the TFIID complex. Adv Protein Chem. 67:67-92. – Matangkasombut O. (2006) Oral Candidiasis Part I : Clinical Manifestations and Etiology. J Dent Assoc Thai. 56 (1): 52-63 – Matangkasombut O. (2006) Oral Candidiasis Part II : Current therapeutic guidelines. J Dent Assoc Thai. 56 (1): 64-75 – Matangkasombut O., Siraprapa J., and Wongpraparat I. (2006) The development of...

Name: Assoc. Prof. Dr. Rojana Sukchawalit Office address: Laboratory of Biotechnology 54 Kamphaeng Phet 6 Talat Bang Khen, Lak Si, Bangkok 10210, Thailand Tel: +66 25530 8555 (ext. 3250) E-mail: [email protected] Research Scientist Research Experience (Specialties) Molecular Microbiology Education 2000-2003 Ph. D. (Biosciences) The University of Birmingham 1993-1996 M. Sc. (Biochemistry) Mahidol University 1990-1993 B. Sc. (Medical Technology) Chulalongkorn University Awards 2012 TRF-CHE-Scopus Young Researcher Award in Life Sciences & Agricultural Sciences Selected Publications Regulation of the Cobalt/Nickel Efflux Operon dmeRF in Agrobacterium tumefaciens and a Link between the Iron-Sensing Regulator RirA and Cobalt/Nickel Resistance. Dokpikul T, Chaoprasid P, Saninjuk K, Sirirakphaisarn S, Johnrod J, Nookabkaew S, Sukchawalit R, Mongkolsuk S. Appl Environ Microbiol. 2016 Jul 15;82(15):4732-42. doi: 10.1128/AEM.01262-16. Agrobacterium tumefaciens Zur Regulates the High-Affinity Zinc Uptake System TroCBA and the Putative Metal Chaperone YciC, along with ZinT and ZnuABC, for Survival under Zinc-Limiting Conditions. Chaoprasid P, Dokpikul T, Johnrod J, Sirirakphaisarn S, Nookabkaew S, Sukchawalit R, Mongkolsuk S. Appl Environ Microbiol. 2016 May 31;82(12):3503-14. doi: 10.1128/AEM.00299-16. Roles of Agrobacterium tumefaciens C58 ZntA and ZntB and the transcriptional regulator ZntR in controlling Cd2+/Zn2+/Co2+ resistance and the peroxide stress response. Chaoprasid P, Nookabkaew S, Sukchawalit R,Mongkolsuk S. Microbiology. 2015 Sep;161(9):1730-40. doi: 10.1099/mic.0.000135. Control of zinc homeostasis in Agrobacterium tumefaciens via zur and the zinc uptake genes znuABC and zinT. Bhubhanil S,...

Name: Dr. James M. Dubbs Office address: Laboratory of Biotechnology 54 Kamphaeng Phet 6 Talat Bang Khen, Lak Si, Bangkok 10210, Thailand Tel: +66 25530 8555 (ext. 3250) E-mail: Research Scientist Research Experience (Specialties) 2002-present Research Scientist, Laboratory of Biotechnology, Chulabhorn Research Institute, Lak Si, Bangkok, Thailand — Supervisor: Prof. Skorn Mongkolsuk 1992-2002 Post-Doctoral Research Associate, The Department of Microbiology, The Ohio State University, Columbus, OH, USA 1990-1991 Post-Doctoral Research Assistant, The Department of Biochemistry, Imperial College of Science, Technology and Medicine, London, UK 1985-1990 Graduate Research Assistant, Intercollegiate Program in Genetics, The Pennsylvania State University, University Park, PA, USA 1983-1985 Research Technician, The Department of Molecular and Cellular Biology, The Pennsylvania State University, University Park, PA, USA Education Ph.D. (Genetics) The Pennsylvania State University, University Park, PA, USA,. (December, 1990) B.S. (Microbiology) The Pennsylvania State University, University Park, PA, USA (May, 1983) Training Workshop 1991 Molecular Genetics of Bacillus thuringiensis at Institute of Biological Control, Faculty of Agriculture, Kyushu University, Japan. 1993 Amino acid sequencer and DNA sequencer, Biosystem Japan, Tokyo, Japan 1998 Plant Molecular Genetics, Department of Genetics, Ghent University, Ghent, Belgium. 2000 Bioremediation, AOTS, Yokohama, Japan Publications – Dubbs, J.M. and S. Mongkolsuk. 2012. Peroxide Sensing Transcriptional Regulators in Bacteria. J. Bacteriol. 194(20): 5495-5503.- Sallabhan, R., J. Kerdwong, J. M. Dubbs, K. Somsongkul, W. Whangsuk, P. Piewtongon,...

Name: Dr. Paiboon Vattanaviboon, Ph.D. Office address: Research Laboratory of Biotechnology, Chulabhorn Research Institute 54 Kamphaeng Phet 6, Talat Bang Khen, Lak Si, Bangkok 10210, Thailand Tel: +662-5538377 (office), +66 831790105 (mobile) E-mail: [email protected] Positions: Research Scientist 2, Research Laboratory of Biotechnology, Chulabhorn Research Institute Research Interests Oxidative stress response in pathogenic bacteria Antimicrobial resistance (AMR) mechanisms in bacteria The impact of antibiotic use in agriculture on AMR Education and Degrees:  1979-1983 B.Sc. (Medical Technology, Hons) Mahidol University 1983-1985 M.Sc. (Microbiology) Mahidol University 1996-1999 Ph.D. (Biotechnology) Mahidol University Working Experience 1985-1986: Research Assistant Department of Biotechnology Faculty of Science, Mahidol University 1986-1991: Scientist Department of Microbiology Army Institute of Pathology 1991-present: Research Scientist ...

CRI’s Institute Animal Care and Use Committee (IACUC) is the oversight body for monitoring and enforcing all animal care and use activities in accordance with CRI’s Animal Care and Use Program (ACUP), policies and standard operating procedures, as well as principles and guidelines in Animals for Scientific Purposes Act, Guide for the Care and Use of Laboratory Animals, 8th ed. (US National Research Council) and the Ethical Principles and Guidelines for the Use of Animals for Scientific Purposes (National Research Council of Thailand). The committee comprises CRI animal researchers, non-laboratory animal researchers, veterinarians, statistician, non-scientists and non-affiliated. New IACUC members must attend a minimum of at least one IACUC training program. All IACUC members are encouraged to attend the aforementioned training once every two years thereafter. Continued IACUC training/updating, in the form of relevant articles and discussions, is conducted during IACUC meetings. The Role of the IACUC include reviews the animal care and use program and conducts animal facility inspections, review animal use protocols and significant changes to the use of animals in ongoing activities, monitor post approval animal protocols and prepare reports to the IO of the IACUC evaluations and recommendations.The IACUC generally meets once a month but no less than once every 6 months. The frequency depends, in part,...

Most antivenoms (AVs) are obtained from the immunization of a large animal (e.g. horse) with only one single venom which resulting to the specific against only a venom, namely monospecific AVs. In case of this AVs, we need to know accurate identification of the culprit snake be bitten. Thus, misdiagnosis could lead to treatment failure and wastage of the expensive AV. To solve this issue, the production of polyspecific antivenom by the immunization with a mixture of venoms from different species of snakes would be useful. Until now, the research on venoms has been continuously developed the efficiency and high dose potency AVs by combination of conventional immunology and advances technology. Therefore, proteomic approach is required for exploration of venom profiles or investigation of the neutralizing toxin by AVs. The challenge of generating effective AVs is universal AV with coverage of all type snakes in a wide geographic area and high efficiency. Furthermore, such AVs would be produced cheaply as a result of economy of scale.