Name: Assoc. Prof. Dr. Panida Navasumrit Office address: Environmental Toxicology Laboratory, Biomedical Building 54 Kamphaeng Phet 6 Talat Bang Khen, Lak Si, Bangkok 10210, Thailand Tel: +66 2553 8555 (ext. 8672) E-mail:

Snake venoms are produced by snakes for digestion and to rapidly immobilize their prey. Venoms contain various hydrolytic enzymes and toxins e.g., neurotoxins and cytotoxins. Neurotoxic venoms are mainly produced by elapid snakes (cobra, krait, mamba) and sea snakes. These toxins cause neuromuscular blockage, leading to respiratory failure and death. Postsynaptic neurotoxins (PSNTs) from different elapids, although chemically and pharmacologically similar, are immunochemically distinct, so antivenom (AV) against venom from one elapid usually fails to neutralize venom of another elapid, even from the same genus. Thus, each country or region needs to produce its own AV, which is not economically or technically always possible. The lack of effective AVs has resulted in numerous deaths of snakebite victims. Our aim is to produce a pan–specific antivenom against venoms of Southeast Asian elapid snakes. Previously we have established an immunization protocol (WHO Guidelines, 2010), which is highly effective in producing high titer and high affinity antibody against PSNTs of various snakes. Potent polyspecific antivenom has been produced against 3 elapids from Thailand with ED50s comparable to the corresponding monospecific antivenoms. Using this immunization protocol with selected neurotoxins from various elapids, we are studying the preparation of a pan-specific antivenom against elapids of SE Asian countries. If successful, this antivenom could be produced for wide distribution and, with economy of scale, should...

Recently, there is an urgent need for rapid diagnostic tests for new infectious agents in man and animals. Conventionally, these infectious agents are identified either by culture or by using molecular biology techniques, e.g. PCR. These processes are costly and usually take several hours or even days. We have produced luminol encapsulated biotinylated liposome and used it as a generic signal generator, capable of conjugation to biotinylated biomolecules, such as specific microbial oligonucleotides, antibody and antigen. The system has been used to detect specific oligonucleotide hybridization and specific antigen-antibody interaction, and the results have been published.

Human pythiosis is an emerging and life-threatening infectious disease caused by the fungus-like organism Pythium insidiosum. It is endemic in Thailand where about 80% of the cases are reported. The disease has high rate of morbidity and mortality, due to lack of early diagnosis and effective treatment. We have developed a convenient and reliable immunochromatographic test (ICT) for rapid detection of human anti-P.insidiosum IgG, for use in early diagnosis and monitoring of pythiosis patients, resulting in saving of limbs and lives. The test kit has been mass produced for free distribution to 6 hospitals with medical schools throughout the country. This research has been published and a patent application has been submitted. In collaboration with researchers from the Department of Pathology, Ramathibodi Hospital, the ICT has been used to study the seroprevalence of P.insidiosum infection in high-risk hematological patients, and a multi-host ICT for the detection of P.insidiosum infection in human, horse and dog has been developed.  

Development of immunochromatographic tests for simple diagnosis of human gnathostomiasis caused by Gnathostoma spinigerumGnathostomiasis is a parasitic infection caused by the third stage larvae of the helminthes Gnathostoma spp., and is found mostly in tropical and subtropical regions. It is a food-borne zoonosis and is endemic in areas where people eat raw freshwater fish or shellfish, especially Thailand and other parts of Southeast Asia, Japan, and increasingly Latin America, particularly Mexico. The common clinical features include an intermittent cutaneous or subcutaneous migratory swelling that may persist for many years. In some cases, the parasite can enter other tissues such as the liver, and the eye, resulting in vision loss or blindness, and the nerves, spinal cord, or brain, resulting in nerve pain, paralysis, coma and death.In this project, we propose to study to produce a recombinant protein from a specific antigen and/or synthesize a specific peptide which will be used as a specific antigen of G. spinigerum and to develop a highly specific and sensitive immunochromatographic test for gnathostomiasis.  

Name: Mrs. Supornrat Pulleium Office address: Research Laboratory of Chemical Carcinogenesis, Chulabhorn Research Institute, 54 Kamphaeng Phet 6 Talat Bang Khen, Lak Si, Bangkok 10210, Thailand Tel: +66 25530 8555 (ext. 3130) E–mail:[email protected] Position: Research Scientist Education and Degreees B.Sc. (Medical technology), Mahidol University, 1988–1991 M.Sc. (Microbiology), Mahidol University, 1992–1994 Work Activities Research Scientist,Laboratory of Chemical Carcinogenesis, Chulabhorn Research Institute, 1995–present

Name: Miss Somkid Sitthimonchai Office address: Research Laboratory of Chemical Carcinogenesis, Chulabhorn Research Institute, 54 Kamphaeng Phet 6 Talat Bang Khen, Lak Si, Bangkok 10210, Thailand Tel: +662–5538555 (ext. 3127-8) (office) Fax:+662–553–8572 Positions:Research Scientist, Research Laboratory of Chemical Carcinogenesis, Chulabhorn Research Institute E-mail:[email protected] Research Interests Biochemistry Education and Degrees B.Sc. (Chemistry), Chiang Mai University, Thailand, 1988-1992 M.Sc. (Biochemistry), Mahidol University, Thailand, 1997-2001 Academic Positions Research Assistant, Chemical Carcinogenesis laboratory, Chulabhorn Research Institute, 1994-1997 Lab Training for Cancer Chemoprevention Screening at German Cancer Research Center (DKFZ), Heidelberg, Germany, 2001-2002 Research Scientist, Chemical Carcinogenesis laboratory, Chulabhorn Research Institute, 2001-present Publications Bhinija, K., Huehne, P. S., Mongkolsuk, S., Sitthimonchai, S., & Satayavivad, J. (2022). A short-chain dehydrogenase/reductase (SDR) detection for the Isoflavone reductase gene in Bulbophyllum and other orchids. South African Journal of Botany, 144, 295-304. doi:https://doi.org/10.1016/j.sajb.2021.08.034 Kheawchaum, S., Mahidol, C., Thongnest, S., Boonsombat, J., Batsomboon, P., Sitthimonchai, S., . . . Prawat, H. (2022). Ent-abietane diterpenoid lactone glycosides and a phenolic glycoside from Phlogacanthus pulcherrimus T. Anderson with cytotoxic and cancer chemopreventive activities. Phytochemistry, 201, 113261. doi:10.1016/j.phytochem.2022.113261 Prachayasittikul, V., Pingaew, R., Worachartcheewan, A., Sitthimonchai, S., Nantasenamat, C., Prachayasittikul, S., . . . Prachayasittikul, V. (2017). Aromatase inhibitory activity of 1,4-naphthoquinone derivatives and QSAR study. EXCLI J, 16, 714-726. doi:10.17179/excli2017-309 Phakeovilay, C., Disadee, W., Sahakitpichan, P., Sitthimonchai, S., Kittakoop, P., Ruchirawat, S., &...

Name: Mister Nirat Pholphana Office address: Research Laboratory of Chemical Carcinogenesis, Chulabhorn Research Institute, 54 Kamphaeng Phet 6 Talat Bang Khen, Lak Si, Bangkok 10210, Thailand Tel: +66 2553 8555 (ext. 3131) Fax:+662-553-8572 E-mail:[email protected] Positions:Research Scientist, Research Laboratory of Chemical Carcinogenesis, Chulabhorn Research Institute Education and Degrees B.Sc. (Medical Technology), Chulalongkorn University, 1991 M.Sc. (Microbiology), Mahidol University, 1995

Name: Dr. Pattama Singhirunnusorn Office address: Research Laboratory of Chemical Carcinogenesis, Chulabhorn Research Institute, 54 Kamphaeng Phet 6 Talat Bang Khen, Lak Si, Bangkok 10210, Thailand Tel: +662–5538555 ext. 3135 (office), +6687–5915431 (mobile) Fax:+662–5538572 Positions:Research Scientist, Research Laboratory of Chemical Carcinogenesis, Chulabhorn Research Institute E–mail:[email protected] Website:– Research Interests Signal transduction Cell and Molecular Biology Education and Degrees: B.Sc. (Chemistry), Suan Dusit Rajabhat University, Bangkok, Thailand, 1994–1997 M.Sc. (Pharmaceutical Chemistry), Mahidol University, Bangkok, Thailand, 1999–2002 Ph.D. (Pharmaceutical Science), University of Toyama, Toyama, Japan, 2002–2005 Academic Positions Researcher, Department of Pharmacognosy, Faculty of pharmacy, Mahidol University, Thailand, 1998 Postdoctoral Fellow, Division of Pathogenic Biochemistry, Department of Bioscience, Institute of Natural Medicine, University of Toyama, Japan, 2006–2008 Researcher, Chemical Carcinogenesis laboratory, Chulabhorn Research Institute, 2008–present Academic Activities Local Activities Project proposal committee (2015–2016), Thesis defend committee (2016–2017), Anti–Aging and Regenerative Medicine Faculty, Dhurakij Pundit University Fellowships Postdoctoral Fellow, Division of Pathogenic Biochemistry, Department of Bioscience, Institute of Natural Medicine, University of Toyama, Japan, 2006–2008 International Awards & Honours Toyama New Industry Organization Grants, Japan, 2006–2007 Monbukagakusho Scholarship, Japan, 2002–2005 Local Awards & Honours Poster award at The 10th National Cancer Conference, Bangkok, Thailand, 2009 ORCID iD: https://orcid.org/0000–0002–8085–5417 Publications A. Research Articles in International Journals Singhirunnusorn, P., Moolmuang, B., Lirdprapamongkol, K., & Ruchirawat, M. (2018). Arsenite exposure potentiates apoptosis–inducing effects of tumor necrosis...

Name: Dr. M.L. Siritida Rabibhadana Office address: Research Laboratory of Chemical Carcinogenesis,Chulabhorn Research Institute, 54 Kamphaeng Phet 6 Talat Bang Khen, Lak Si, Bangkok 10210, Thailand Tel: +662-5538555 ext 3130 Position: Research Scientist E-mail:[email protected] Research Interests:Cell and Molecular Biology Education and Degrees: B. Sc.(Biotechnology)Mahidol University, 1983-1987 M. Sc. (Microbiology)Mahidol University, 1987-1991 Ph. D. (Microbiology)Mahidol University, 1991-1996 Awards Award of Highest Grade Point Average from Dr. Tab Foundation, 1987 Scholarship from the National Center of Genetic Engineering and Biotechnology, 1990 Scholarship from the National Science and Technology Agency, 1991-1996 Work Activities August 1989-October 1989 Molecular Biology Training at University of Maryland, Baltimore County, USA October 1989-April 1990 Molecular Training at Oregon State University, USA September 1998-March 1999 Molecular Training at International Agency for Research on Cancer (IARC), Lyon, France June 28, 2003-July 26, 2003 Microarray Technology Training at Massachusetts Institute of Technology (MIT), Cambridge, MA, USA February 23, 2004-March 31, 2004 Microarray Technology Training at Oregon Health Science Technology (OHSU), Portland, OR, USA November 1, 2004 -January 27, 2005 ELISA Technique Training at National Cancer Institute (NCI), Bethesda, MD, USA Publications Schaffer, A. A., Dominguez, D. A., Chapman, L. M., Gertz, E. M., Budhu, A., Forgues, M., . . . Wang, X. W. (2021). Integration of adeno-associated virus (AAV) into the genomes of most Thai and Mongolian liver cancer patients does...

Name: Dr. Jittiporn Chaisaingmongkol Office address: Research Laboratory of Chemical Carcinogenesis, Chulabhorn Research Institute, 54 Kamphaeng Phet 6 Talat Bang Khen, Lak Si, Bangkok 10210, Thailand Tel: +662-5538555 ext. 3136 (office) Fax:+662-5538572 Position: Research Scientist I, Research Laboratory of Chemical Carcinogenesis, Chulabhorn Research Institute E-mail:[email protected] Research Interests Cancer biology and molecular biology (Epigenetics and DNA repair) Integrated and functional genomics of human liver cancer (Hepatocellular carcinoma and cholangiocarcinoma) Education and Degrees: 2000 – 2006 Diplom Degree (Bachelor and Master degree) Bayerischen Julius-Maximilians-Universitaet Wuerzburg, Wuerzburg, GERMANY 2006 – 2011 Doctor of Science (DR. RER. NAT.) Ruperto-Carola University of Heidelberg, Heidelberg, GERMANY Fellowships 2011 Postdoctoral Fellow at the German Cancer Research Center, Heidelberg, GERMANY 2013-2016 Visiting fellow at Laboratory of Human Carcinogenesis, National Cancer Institute, National Institute of Health, Bethesda, MD, USA ORCID iD: https://orcid.org/0000-0001-8019-5562 Publications Ma L, Heinrich S, Wang L, Keggenhoff FL, Khatib S, Forgues M, et al. Multiregional single-cell dissection of tumor and immune cells reveals stable lock-and-key features in liver cancer. Nat Commun. 2022;13(1):7533. Khatib SA, Ma L, Dang H, Forgues M, Chung JY, Ylaya K, et al. Single-cell biology uncovers apoptotic cell death and its spatial organization as a potential modifier of tumor diversity in HCC. Hepatology. 2022;76(3):599-611. Schaffer AA, Dominguez DA, Chapman LM, Gertz EM, Budhu A, Forgues M, et al. Integration of adeno-associated virus (AAV)...

Name: Dr. Benchamart Khumkrong Office address: Research Laboratory of Chemical Carcinogenesis, Chulabhorn Research Institute, 54 Kamphaeng Phet 6 Talat Bang Khen, Lak Si, Bangkok 10210, Thailand Tel: +662–5538555 ext. 3136 (office), +6680–9001736 (mobile) Fax:+662–553–8572 Positions:Research Scientist I, Research Laboratory of Chemical Carcinogenesis, Chulabhorn Research Institute E–mail:[email protected] Website: Research Interests Cancer biology Cell and molecular biology Cholangiocarcinoma Education and Degrees: B.Sc. Biology, Khon Kaen University, Thailand, 2001 M.Sc. Molecular Genetics and Genetic Engineering, Mahidol University, Thailand, 2004 Ph.D. Cancer Biology Program, Wayne State University, USA, 2010 Academic Positions Research Assistant, Genomic Lab, Mahidol University, 2004–2005 Research Associate, Tainsky Lab, Wayne State University, 2010 Researcher Scientist I, Chemical Carcinogenesis Laboratory, Chulabhorn Research Institute, 2010–present Local Awards & Honours The Development and Promotion of Science and Technology Talent Project Scholarship granted by the Ministry of Science and Technology, Thailand, 1998–2004 The Thai Government Science and Technology Scholarship for Doctoral study granted by the Office of the Civil Service Commission, Thailand, 2005–2010 A New Researcher Scholarship of Coordinating Center for Thai Government Science and Technology Scholarship Students (CSTS), 2011–2012 ORCID iD : https://orcid.org/0000–0003–2008–4021 Publications Research Articles in International Journals Moolmuang, B., & Ruchirawat, M. (2021). The antiproliferative effects of ataxia–telangiectasia mutated and ATM– and Rad3–related inhibitions and their enhancements with the cytotoxicity of DNA damaging agents in cholangiocarcinoma cells. J Pharm Pharmacol, 73(1), 40–51....