Name: Miss Pantipa Subhasitanont Office address: B704B, 7th Floor, Biomedical Science Building 54 Kamphaeng Phet 6 Talat Bang Khen, Lak Si, Bangkok 10210, Thailand Tel: +66 2553 8555 (ext. 3241) E-mail: [email protected] Research Scientist Education – B.Sc. (Biotechnology) Mahidol University, Bangkok, Thailand – M.Sc. (Biochemistry) Mahidol University, Bangkok, Thailand Publications 1. Keeratichamroen S, Subhasitanont P, Chokchaichamnankit D, Weeraphan C, Saharat K, Sritana N, Kantathavorn N, Wiriyaukaradecha K, Sricharunrat T, Paricharttanakul NM, Auewarakul C, Svasti J, Srisomsap C. Identification of potential cervical cancer serum biomarkers in Thai patients. Oncol Lett. 2020; 19(6): 3815-3826. 2. Chokchaichamnankit D, Watcharatanyatip K, Subhasitanont P, Weeraphan C, Keeratichamroen S, Sritana N, Kantathavorn N, Diskul-Na-Ayudthaya P, Saharat K, Chantaraamporn J, Verathamjamras C, Phoolcharoen N, Wiriyaukaradecha K, Paricharttanakul NM, Udomchaiprasertkul W, Sricharunrat T, Auewarakul C, Svasti J, Srisomsap C. Urinary biomarkers for the diagnosis of cervical cancer by quantitative label-free mass spectrometry analysis. Oncol Lett. 2019; 17(6): 5453-5468. 3. Subhasitanont P, Chokchaichamnankit D, Chiablaem K, Keeratichamroen S, Ngiwsara L, Paricharttanakul NM, Lirdprapamongkol K, Weeraphan C, Svasti J, Srisomsap C. Apigenin inhibits growth and induces apoptosis in human cholangiocarcinoma cells. Oncol Lett. 2017; 14(4): 4361-4371. 4. Verathamjamras C, Weeraphan C, Chokchaichamnankit D, Watcharatanyatip K, Subhasitanont P, Diskul-Na-Ayudthaya P, Mingkwan K, Luevisadpaibul V, Chutipongtanate S, Champattanachai V, Svasti J, Srisomsap C. Secretomic profiling of cells from hollow fiber bioreactor reveals...

Name: Narittira Ornnork Office address: B704A, 7th Floor, Biomedical Science Building 54 Kamphaeng Phet 6 Talat Bang Khen, Lak Si, Bangkok 10210, Thailand Tel: +66 2553 8555 (ext. 8349) Mobile: 0982716213 E-mail: [email protected] Research Assistant Research Interests Cancer biology, Cell signaling, Drug resistance, Drug discovery, Apoptosis Education – B.Sc. (Biotechnology), King Mongkut’s University of Technology North Bangkok, THAILAND Publications 1. Ornnork, N., Kiriwan, D., Lirdprapamongkol, K., Choowongkomon, K., Svasti, J., & Eurtivong, C. (2020). Molecular dynamics, MM/PBSA and in vitro validation of a novel quinazoline-based EGFR tyrosine kinase inhibitor identified using structure-based in silico screening. Journal of Molecular Graphics and Modelling, 99, 107639. 2. Saruengkhanphasit, R., Butkinaree, C., Ornnork, N., Lirdprapamongkol, K., Niwetmarin, W., Svasti, J., & Eurtivong, C. (2021). Identification of new 3-phenyl-1H-indole-2-carbohydrazide derivatives and their structure–activity relationships as potent tubulin inhibitors and anticancer agents: A combined in silico, in vitro and synthetic study. Bioorganic Chemistry, 110, 104795. 3. Keeratichamroen, S., Lirdprapamongkol, K., Thongnest, S., Boonsombat, J., Chawengrum, P., Sornprachum, Ornnork, N., & Svasti, J. (2022). JAK2/STAT3‑mediated dose‑dependent cytostatic and cytotoxic effects of sesquiterpene lactones from Gymnanthemum extensum on A549 human lung carcinoma cells. Oncology Reports, 47(1), 1-11.

Name: Dr. Lukana Ngiwsara Office address: B701C, 7th Floor, Biomedical Science Building 54 Kamphaeng Phet 6 Talat Bang Khen, Lak Si, Bangkok 10210, Thailand Tel: +66 2553 8555 (ext. 3237-8) E-mail: [email protected] Position Research Scientist I Research interests Human genetic disease Cancer biology Education – B.Sc. (Biochemistry), Chulalongkorn University, Thailand – M.S. (Biochemistry), Mahidol University, Thailand – M.S. (Agrobiology), Hokkaido University, Japan – Ph.D. (Agrobiology), Hokkaido University, Japan Scholarships 2000-2002 Royal Golden Jubilee (Chulabhorn foundation) 2007-2012 Japanese Government Scholarship (MEXT) Publications – Anurathapan U, Tim-Aroon T, Zhang W, Sanpote W, Wongrungsri S, Khunin N, Chutipongtanate S, Chirdkiatgumchai V, Ngiwsara L, Jaovisidha S, Khongkraparn A, Pakakasama S, Svasti J, Setchell KDR, Wattanasirichaigoon D, Hongeng S. Comprehensive and long-term outcomes of enzyme replacement therapy followed by stem cell transplantation in children with Gaucher disease type 1 and 3. Pediatr Blood Cancer. 2023 Mar;70(3):e30149. doi: 10.1002/pbc.30149. Epub 2022 Dec 23. – Ngiwsara L, Sawangareetrakul P, Wattanasirichaigoon D, Tim-Aroon T, Dejkhamron P, Champattanachai V, Ketudat-Cairns JR, Svasti J. Effects of gentamicin inducing readthrough premature stop Codons: A study of alpha-L-iduronidase nonsense variants in COS-7 Cells. Biochem Biophys Res Commun. 2022 Oct 27;636(Pt 1):147-154. doi: 10.1016/j.bbrc.2022.10.081. – Chaiputtanapun P, Lirdprapamongkol K, Thanaussavadate B, Phongphankhum T, Thippong T, Thangsan P, Montatip P, Ngiwsara L, Svasti J, Chuawong P. Biphasic Dose-Dependent G0/G1 and G2/M Cell-Cycle Arrest by...

Name: Dr. Kriengsak Lirdprapamongkol Office address: Laboratory of Biochemistry, 7th floor, Biomedical Science Building Chulabhorn Research Institute, 54 Kamphaeng Phet 6 Talat Bang Khen, Lak Si, Bangkok 10210, Thailand Tel: +66 2553 8555 (ext. 3239) Mobile: +66-86-986-4485 Fax: +66-2-553-8572 E-mail: [email protected] Research Scientist II Research Interests Cancer biology, Metastasis, Drug resistance, Hypoxia, Apoptosis, Cell signaling, Drug discovery, Photodynamic therapy, Sonodynamic therapy, Natural products Education – B.Sc. (Biotechnology), King Mongkut’s Institute of Technology Ladkrabang, THAILAND – M.Sc. (Biochemistry), Mahidol University, THAILAND – Ph.D. (Biochemistry), Mahidol University, THAILAND Publications Journal 1. Thongnest S, Boonsombat J, Keeratichamroen S, Lirdprapamongkol K, Kaweetripob W, Kheawchaum S, et al. Acetogenins from the stems of Uvaria micrantha showing antiproliferative effects on HepG2 liver cancer cells. Phytochemistry. 2022;204:113450. 2. Keeratichamroen S, Lirdprapamongkol K, Thongnest S, Boonsombat J, Chawengrum P, Sornprachum T, et al. JAK2/STAT3mediated dosedependent cytostatic and cytotoxic effects of sesquiterpene lactones from Gymnanthemum extensum on A549 human lung carcinoma cells. Oncol Rep. 2022;47(1). 3. Chaiputtanapun P, Lirdprapamongkol K, Thanaussavadate B, Phongphankhum T, Thippong T, Thangsan P, et al. Biphasic Dose-Dependent G0/G1 and G2/M Cell-Cycle Arrest by Synthetic 2,3-Arylpyridylindole Derivatives in A549 Lung Cancer Cells. ChemMedChem. 2022;17(14):e202200127. 4. Saruengkhanphasit R, Butkinaree C, Ornnork N, Lirdprapamongkol K, Niwetmarin W, Svasti J, et al. Identification of new 3-phenyl-1H-indole-2-carbohydrazide derivatives and their structure-activity relationships as potent tubulin inhibitors and anticancer agents:...

Name: Miss Daranee Chokchaichamnankit Office address: B704B, 7th Floor, Biomedical Science Building, 54 Kamphaeng Phet 6 Talat Bang Khen, Lak Si, Bangkok 10210, Thailand Tel: +66 25530 8555 (ext. 3235, 8530) E-mail: [email protected] Research Scientist Research Interests Protein purification and protein assay, Cancer proteomics Education B.Sc. Chemistry, Ramkhamhaeng University, Bangkok, THAILAND M.Sc. Chemistry, Kasetsart University, Bangkok, THAILAND Publications 1. Nonthawong K., Srisomsap C., Chokchaichamnankit D., Svasti J., Phiriyangkul P. Comparative proteomics and in silico allergenicity of fresh and powdered skipjack tuna and Nile tilapia. Food Control. Volume 144, February 2023, 109345. https://doi.org/10.1016/j.foodcont.2022.109345 2. Watcharatanyatip K., Chutipongtanate S., Chokchaichamnankit D., Weeraphan C., Mingkwan K., Luevisadpibul V., Newburg D.S., Morrow A.L., Svasti J. and Srisomsap C. Translational Proteomic Approach for Cholangiocarcinoma Biomarker Discovery, Validation, and Multiplex Assay Development: A Pilot Study. Molecules. 27(18): 5904, 2022. https://doi.org/10.3390/molecules27185904 3. Komkleow S., Weeraphan C., Chokchaichamnankit D., Chaisuriya P., Verathamjamras C., Ruangjaroon T., Svasti J., Sangvanich P. and Srisomsap C. Global Analysis of Protein Expression in A549 Cells After Prolonged Nicotine Exposure by Using Label-free Quantification. Anticancer Res. 41(8):3833-3842, 2021. doi: 10.21873/anticanres. 15176. PMID: 34281843 4. Mon M.M., Srisomsap C., Chokchaichamnankit D., Watcharatanyatip K., Weeraphan C., Svasti J., Maneechai K., Thongsuksai P. and Raungrat P. Expressed IGHG3 and A1AG1 as Potential Predictors of Chemotherapeutic Response in Advanced Non-small Cell Lung Cancer. Anticancer Res. 41(4):1871-1882, 2021. doi:...

Name: Dr. Churat Weeraphan Office address: B707C, 7th Floor, Biomedical Science Building 54 Kamphaeng Phet 6 Talat Bang Khen, Lak Si, Bangkok 10210, Thailand Tel: +66 2553 8555 (ext. -) Mobile: 081-170-7570 E-mail: [email protected] Research Scientist Research Interests Current research interest is focused on understanding the molecular function of nano-size extracellular vesicles using Integrative Multi-Omics Approaches. Education – B.Sc. (Agriculture), King Mongkut’s Institute of Technology Ladkrabang, THAILAND – M.Sc. (Biochemistry), Mahidol University, THAILAND – Ph.D. (Molecular Biology and Bioinformatics), Prince of Songkla University, THAILAND Publications 1. Weeraphan, C., Diskul-Na-Ayudthaya, P., Chiablaem, K., Khongmanee, A., Chokchaichamnankit, D., Subhasitanont, P., Svasti, J., and Srisomsap, C. (2012) Effective enrichment of cholangiocarcinoma secretomes using the hollow fiber bioreactor culture system. Talanta 99, 294-301 2. Weeraphan, C., Srisomsap, C., Chokchaichamnankit, D., Subhasitanont, P., Hatairaktham, S., Charoensakdi, R., Panichkul, N., Siritanaratkul, N., Fucharoen, S., Svasti, J., and Kalpravidh, R. W. (2013) Role of curcuminoids in ameliorating oxidative modification in beta-thalassemia/Hb E plasma proteome. J Nutr Biochem 24, 578-585 3. Chutipongtanate, S., Changtong, C., Weeraphan, C., Hongeng, S., Srisomsap, C., and Svasti, J. (2015) Syringe-push membrane absorption as a simple rapid method of urine preparation for clinical proteomics. Clin Proteomics 12, 15 4. Chaiyawat, P., Weeraphan, C., Netsirisawan, P., Chokchaichamnankit, D., Srisomsap, C., Svasti, J., and Champattanachai, V. (2016) Elevated O-GlcNAcylation of Extracellular Vesicle Proteins Derived from...

Name: Chris Verathamjamras Office address: B705A, 7th Floor, Biomedical Science Building 54 Kamphaeng Phet 6 Talat Bang Khen, Lak Si, Bangkok 10210, Thailand Tel: +66 2553 8555 (ext. 3230) E-mail: [email protected] Research Scientist I Education – B.Sc. (Agriculture), King Mongkut’s Institute of Technology Ladkrabang, THAILAND – M.Sc. (Microbiology), Mahidol University, THAILAND Publications 1. Siriporn Keeratichamroen, Kriengsak Lirdprapamongkol, Sanit Thongnest, Jutatip Boonsombat, Pornsuda Chawengrum, Thiwaree Sornprachum, Jitnapa Sirirak, Chris Verathamjamras, Narittira Ornnork, Somsak Ruchirawat and Jisnuson Svasti. JAK2/STAT3‑mediated dose‑dependent cytostatic and cytotoxic effects of sesquiterpene lactones from Gymnanthemum extensum on A549 human lung carcinoma cells. Oncol Rep. 2022 Jan;47(1):6. doi: 10.3892/or.2021.8217. Epub 2021 Nov 5. 2. Sasikarn Komkleow, Churat Weeraphan, Daranee Chokchaichamnankit, Papada Chaisuriya, Chris Verathamjamras, Theetat Ruangjaroon, Jisnuson Svasti, Polkit Sangvanich and Chantragan Srisomsap. Global Analysis of Protein Expression in A549 Cells After Prolonged Nicotine Exposure by Using Label-free Quantification. Anticancer Res. 2021 Aug;41(8):3833-3842. doi: 10.21873/anticanres.15176. 3. Verathamjamras C*, Sriwitool T*, Netsirisawan P, Chaiyawat P, Chokchaichamnankit D, Prasongsook N, Srisomsap C and Svasti J, Champattanachai V. Aberrant RL2 O-GlcNAc antibody reactivity against serum-IgA1 of patients with colorectal cancer. Glycoconj J. 2021 Feb;38(1):55-65. doi: 10.1007/s10719-021-09978-8. Epub 2021 Feb 20. 4. Chantaraamporn J, Champattanachai V, Khongmanee A, Verathamjamras C, Prasongsook N, Mingkwan K, Luevisadpibul V, Chutipongtanate S and Svasti J. Glycoproteomic Analysis Reveals Aberrant Expression of Complement C9 and Fibronectin in the...

Name: Emeritus Professor M.R. Jisnuson Svasti Office address: Laboratory of Biochemistry, Chulabhorn Research Institute, 54 Kamphaeng Phet 6 Talat Bang Khen, Lak Si, Bangkok 10210, Thailand Tel: +662-5538561 (office), +6681-8212135(mobile) Fax:+662-259-6634 Positions:Director, Laboratory of Biochemistry, Chulabhorn Research Institute, and Emeritus Professor of Biochemistry, Mahidol University, Bangkok E-mail:[email protected],[email protected],[email protected] Website: https://science.mahidol.ac.th/expertise/search.php?q=Jisnuson%20Svasti Research Interests General interests in studying structure-function relationships in proteins and enzymology. Past experiences include study of Plasma Proteins, Proteins unique to the male reproductive system, Abnormal hemoglobins. Current research interests include: Inborn errors of metabolism; Protein changes in cancer, anti-cancer agents from natural products; Cancer Biology; Drug delivery: Glycosidase enzymes and glycosides Education:Educated in England (1954-1972) • Cheam School, Headley, Nr. Newbury, Berkshire, 1957-1960 • Rugby School, Rugby, Warwickshire, 1960-1965 • Trinity College, Cambridge University, 1965-1972 Degrees: • B.A.(Hons) Natural Sciences (Biochemistry), University of Cambridge, U.K., 1968 • M.A., Natural Sciences, University of Cambridge, U.K. 1972 • Ph.D., MRC Laboratory of Molecular Biology, University of Cambridge, U.K. Thesis title: Sequence Studies on Mouse Immunoglobulins; Supervisor: Dr..Cesar Milstein, F.R.S. (Nobel laureate), 1972 • D.Sc. (Honoris causa), Biochemistry, Mahidol University, Thailand, 2013 • D.Sc. (Honoris causa), Biochemistry, Suranaree University of Technology, Thailand, 2015 Academic Positions Lecturer (1972-1975), Assistant Professor (1975-1978), Associate Professor (1979-1982), Professor (1982-2002), Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, Senior Research Associate, Department of Human Biological Chemistry...

Photodynamic therapy (PDT) has proven to be an effective and minimally invasive modality for cancer treatment, which can be used alone or together with other conventional treatments. PDT requires three harmless key components: photosensitizer, light and oxygen. The treatment protocol is based on the activation of a photosensitizer by using a suitable wavelength of light in the presence of tissue oxygen, which then results in the generation of singlet oxygen or other reactive oxygen species (ROS) to destroy cancer cells. PDT also offers significant benefits to overcome a variety of cancers. Many photosensitizers are hydrophobic and show tendency to aggregate in aqueous solutions, so that encapsulation in drug delivery system may improve their PDT performance. Recently, our laboratory successfully prepared polymer-lipid hybrid nanoparticles with good biocompatibility to deliver the hydrophobic photosensitizers that expand the therapeutic applications of PDT to multidrug resistant (MDR)lung cancer cells. The formulated nanoparticles have the potential to improve the solubility of photosensitizer. Also, nanoparticles may augment in vitro PDT efficacy by effectively increasing the accumulation of photosensitizer in MDR cells without being affected by the MDR mechanisms, leading to enhanced light-induced generation of singlet oxygen and superoxide anion, which finally causes apoptotic cell death. Cancer immunotherapy is another area of interest. Some cancer cells have partner proteins that bind to...

It is now widely accepted that the tumor microenvironment influences the fate and behavior of cancer cells and plays crucial roles in regulating tumor progression and therapeutic responses. The microenvironment of cells cultured in vitro on traditional plastic substrata does not account for cell interactions with the extracellular matrix (ECM) and is thus a less reliable approach to analyze cellular activity. However, most studies are performed with cancer cells grown in two-dimensions on plastic plates, leading to high failure rate of drug discovery and development. Culture conditions that recreate the appropriate microenvironment for cells should provide a better model for studying cellular activity and discovery of anticancer drugs. We thus developed cell culture models that more closely resemble in vivo tumor biology and investigated its consequences on cellular activity and responses to anticancer compounds. The results of an in vitro 3D model showed that 3D cultured cells on Matrigel showed cellular dormancy (G0/G1 cell cycle arrest), decreased cell migration and invasion, and were resistant towards a variety of chemotherapeutic agents.Further investigation of the molecular mechanisms implicated in tumor dormancy and drug resistance led to the identification of a key mediator involved in the development of chemoresistance in 3D cultures which may offer the potential of developing effective targeted therapies for cancer.

Metastasis and drug resistance of cancer cells are major problems in cancer therapy. Metastasis is the spread of cancer cells in the body, from primary tumor site to distant organs, where the cancer cells remain dormant for months to years as micrometastases, and then regrow to become recurrent tumors. We have explored the effect of chemicals and drugs on metastasis of cancer cell lines. A common environmental contaminant, perfluorooctanoic acid (PFOA), enhanced the metastatic potential of FTC133 thyroid cancer cells, while sub-lethal doses of the drug, doxorubicin, increased metastatic potential of low-invasive HepG2 liver cancer cells. On the other hand, vanillin, the vanilla flavoring agent, was found to reduce the metastatic potential of 4T1 mouse breast cancer cells and to suppress metastasis in an animal model, orally given vanillin for a month. Vanillin and its structure-related compound, apocynin, decreased metastatic potential of A549 lung cancer cells by inhibiting the PI3K/Akt signaling pathway. Homodimers of vanillin and apocynin were more potent than their monomers in inhibiting invasion of HepG2 liver cancer cells, and protein focal-adhesion kinase (FAK) was identified as a target of the dimeric compounds. Chrysin, found in Thai propolis, also showed anti-metastatic potential by reducing tumor recurrence, through suppressing growth of breast cancer micrometastases after spreading to the lungs of mice.With chrysin, STAT3 inhibition was the mechanism for inhibiting...

Many proteins are chemically modified after synthesis. These Post-Translational Modifications (PTMs), such as phosphorylation and O-GlcNAcylation, play an important role in regulating metabolic processes. Our studies show that the levels of O-GlcNAcylation and O-GlcNAc transferase (OGT), an enzyme catalyzing the addition of a single GlcNAc residue to target proteins, were up-regulated in breast and colon cancer tissues and cell lines. Using mass spectrometry, many O-GlcNAc-modified proteins were identified in these cancers, for example pyruvate kinase M2 (PKM2). O-GlcNAcylation of PKM2 affects its phosphorylation as well as its activity, thus indicating the crosstalk between these two PTMs. We also demonstrated that OGT knockdown led to a significant reduction of colony formation in breast and colon cell lines, suggesting it plays vital roles in tumor progression. Phosphorylation is another regulatory mechanism, but identification of phosphoproteins can sometimes be difficult due to possible digestion by phosphatase enzyme. We have therefore isolated exosomes, extracellular vesicles (EVs) of 30-100 nm size, from the conditioned media of isogenic cholangiocarcinoma cell lines with different metastatic potential. More than 40 phosphoproteins were identified with significant change in phosphorylation level. Heat Shock Protein 90 was confirmed as showing differential phosphorylation in relation to tumor invasiveness, so aberrant phosphorylation of exosomal proteins may be useful for development of a metastatic cancer biomarker. Indeed, since EVs contain many functional biomolecules, such...