Chulabhorn Research Institute had been collaborating with Chulabhorn hospital, the National Cancer Institute (Thailand), Khon Kaen University, and Chiang Mai University with the aim to alleviate the suffer of liver cancer patients and the tumor biobank for liver cancer was set up to provide resource for future investigations. Since 2008, CRI, participating Thai institutions and the National Cancer Institute (Bethesda, USA) has established the Thailand Initiative on Genomics and Expression Research for Liver Cancer (TIGER-LC) consortium. The goal is to perform a comprehensive large scale genome-wide and candidate association study to identify and validate genome factors and tumor biology that affect liver cancer risk progression and response to therapy. The MOU signing ceremony of TIGER-LC between CRI and collaborators The study aims to include 6,000 volunteers which are 1,000 HCC cases; 2,000 CCA cases, 2,000 patients with chronic liver diseases arising from hepatitis B and or hepatitis C infection, alcoholic cirr hosis, and liver fluke carriers which are considered high risk non-carrier cases, as well as a sample of 1,000 healthy population-based controls recruited at our collaborating institutions and Chulabhorn hospital. Information from questionnaire, clinical data and specimens such as blood, urine, saliva and tissue were collected with the same efficiency and standard and delivered to CRI. The biobank and database system are set up at CRI to accommodate a...

The main focus of the research is to understand the relationship among genetics, environmental exposures, and the etiology of primary liver cancer through the establishment of a large clinical cohort consisting of well-defined case-control and case-case individuals. Recognizing the molecular features and genetic mutations of liver cancer from Thai patients could lead to more effective treatment options for this population. These findings may point researchers toward new strategies for drug development. Through integrative analysis approaches, the research also aims to identify clinically-relevant biomarkers that may be useful for risk assessment, cancer early detection, tumor subtype classification, prediction of tumor relapses and responses to therapy which could potentially lead to more effective treatment of liver cancers. Using biospecimens collected in the biorepository, the researchers studied multi-omics data arising from tissue of hepatocellular carcinoma (HCC) and cholangiocarcinoma (CCA) patients from Thailand. Genome sequences, gene activity and metabolic profiles data of 398 tissue samples were analyzed and the researchers were able to identify molecular subtypes of either type of liver cancer which are closely resembles each other’s. These common subtypes of HCC and CCA showed similar gene expression pattern, tumor biology and clinical outcome. The follow up study focused on metabolic profile of Thai HCC patients, the researchers discovered that certain metabolic genes, tissue metabolites and serum metabolites can independently stratify HCC patients...

Cholangiocarcinoma (CCA) is malignancy arising from cholangiocyte in the biliary tract and is the second most common type of primary liver cancer. This type of cancer is associated with poor prognosis and limited treatment options. The major risk factors of this cancer associate with chronic inflammation, cellular injury in the bile duct, and partial obstruction of bile flow caused by various conditions such as primary sclerosing cholangitis (PSC), hepatolithiasis and infestation by liver fluke (Opisthorchis viverrini) (Kubo, Kinoshita, Hirohashi, & Hamba, 1995; Sirica, 2005; Watanapa & Watanapa, 2002). Recent studies attempt to investigate the molecular pathogenesis of this disease, including etiology and molecular alterations. Genetics and epigenetics changes in cholangiocarcinoma have been identified from a number of studies. Alterations of several cellular functions including self-sufficiency and proliferation, apoptosis resistance, escape from senescence, and tumor invasiveness and metastasis, are proposed to contribute the onset of cholangiocarcinoma (Lazaridis & Gores, 2005). The uncontrolled proliferation of cells due to harboring of genes mutations causes cancer development. Cell cycle progression is a highly regulated process that involves several steps including DNA replication and cell mitosis. At present, understanding the mechanisms involved in cholangiocarcinoma cells development and finding the indicators of the response to cancer treatment is beneficial to the treatment option of this cancer. Recent research conducted by the Thailand Initiative in Genomics and...

Name: Jarunee Thuraphan Office address: Research Laboratory of Chemical Carcinogenesis, Chulabhorn Research Institute, 54 Kamphaeng Phet 6 Talat Bang Khen, Lak Si, Bangkok 10210, Thailand Tel: +66 2553 8555 (ext. 3129) (office) Fax:+662–553–8572 Position: Research Assistant, Research Laboratory of Chemical Carcinogenesis, Chulabhorn Research Institute E–mail: [email protected] Education and Degree B.Sc. (Biology), Ramkhamhaeng University, 1998–2004 Work Activities Marketing, General drug house Ltd., 2003–2005 Research Assistant, Research Ethic Committee, Academic Support Department, Rajavithi Hospital, 2005–2010 Research Assistant, Chemical Carcinogenesis laboratory, Chulabhorn Research Institute, 2011–present

Name:Ms. Donlaporn Sriphan Office address: Research Laboratory of Chemical Carcinogenesis, Chulabhorn Research Institute, 54 Kamphaeng Phet 6 Talat Bang Khen, Lak Si, Bangkok 10210, Thailand Tel: +662–5538555 (ext. 3133) Fax:– Positions:Research Scientist, Research Laboratory of Chemical Carcinogenesis, Chulabhorn Research Institute E–mail:[email protected] Website:– Research Interests Microbiome Bioinformatics Cancer Education and Degrees: Thammasat University, 2009 – 2016, Chulalongkorn University, 2013 – 2016 B.Sc. (Biotechnology), Thammasat University, TH., 2012 M.Sc. (Microbiology and microbial technology), Chulalongkorn University, TH. 2016 ORCID iD: https://orcid.org/0000-0003-2883-3357 Publications Sripan, D., Wilantho, A., Khitmoh, K., Wongsawaeng, D., Ouazzani, J., Chavanich, S., Tongsima, S., Somboonna, N. (2021). Marine Bacteria Community in a 150–m Depth Tachai Island, the Southeast Andaman Sea of Thailand. Frontiers in Marine Science, 8. doi:10.3389/fmars.2021.624624  

Name: Miss Buasai Akachart Office address: Research Laboratory of Chemical Carcinogenesis, Chulabhorn Research Institute, 54 Kamphaeng Phet 6 Talat Bang Khen, Lak Si, Bangkok 10210, Thailand Tel: +66 2553- 8555 (ext. 3146) Fax:+662–553–8572 E-mail: [email protected] Position: Research Assistant, Research Laboratory of Chemical Carcinogenesis, Chulabhorn Research Institute Education and Degrees: B.Sc. (Biotechnology), Khon kaen University, 2003-2007 Work Activities Research Assistant, Department of Biotechnology, Faculty of Technology Khon kaen University, 2008-2011 Research Assistant, Chemical Carcinogenesis Laboratory, Chulabhorn Research Institute. Responsible for biobank data depository and initial data quality control steps, 2012-present Publications Yuvadetkun, P., Ackachat, B., & Boonmee, M. (2017). Repetitive dilute acid hydrolysis of sugarcane bagasse for ethanol production by Candida shehatae TISTR 5843. Asia-Pacific Journal of Science and Technology, 18(1), 112-121. Retrieved from https://so01.tci-thaijo.org/index.php/APST/article/view/82820  

Name: Mrs. Supornrat Pulleium Office address: Research Laboratory of Chemical Carcinogenesis, Chulabhorn Research Institute, 54 Kamphaeng Phet 6 Talat Bang Khen, Lak Si, Bangkok 10210, Thailand Tel: +66 25530 8555 (ext. 3130) E–mail:[email protected] Position: Research Scientist Education and Degreees B.Sc. (Medical technology), Mahidol University, 1988–1991 M.Sc. (Microbiology), Mahidol University, 1992–1994 Work Activities Research Scientist,Laboratory of Chemical Carcinogenesis, Chulabhorn Research Institute, 1995–present

Name: Miss Somkid Sitthimonchai Office address: Research Laboratory of Chemical Carcinogenesis, Chulabhorn Research Institute, 54 Kamphaeng Phet 6 Talat Bang Khen, Lak Si, Bangkok 10210, Thailand Tel: +662–5538555 (ext. 3127-8) (office) Fax:+662–553–8572 Positions:Research Scientist, Research Laboratory of Chemical Carcinogenesis, Chulabhorn Research Institute E-mail:[email protected] Research Interests Biochemistry Education and Degrees B.Sc. (Chemistry), Chiang Mai University, Thailand, 1988-1992 M.Sc. (Biochemistry), Mahidol University, Thailand, 1997-2001 Academic Positions Research Assistant, Chemical Carcinogenesis laboratory, Chulabhorn Research Institute, 1994-1997 Lab Training for Cancer Chemoprevention Screening at German Cancer Research Center (DKFZ), Heidelberg, Germany, 2001-2002 Research Scientist, Chemical Carcinogenesis laboratory, Chulabhorn Research Institute, 2001-present Publications Bhinija, K., Huehne, P. S., Mongkolsuk, S., Sitthimonchai, S., & Satayavivad, J. (2022). A short-chain dehydrogenase/reductase (SDR) detection for the Isoflavone reductase gene in Bulbophyllum and other orchids. South African Journal of Botany, 144, 295-304. doi:https://doi.org/10.1016/j.sajb.2021.08.034 Kheawchaum, S., Mahidol, C., Thongnest, S., Boonsombat, J., Batsomboon, P., Sitthimonchai, S., . . . Prawat, H. (2022). Ent-abietane diterpenoid lactone glycosides and a phenolic glycoside from Phlogacanthus pulcherrimus T. Anderson with cytotoxic and cancer chemopreventive activities. Phytochemistry, 201, 113261. doi:10.1016/j.phytochem.2022.113261 Prachayasittikul, V., Pingaew, R., Worachartcheewan, A., Sitthimonchai, S., Nantasenamat, C., Prachayasittikul, S., . . . Prachayasittikul, V. (2017). Aromatase inhibitory activity of 1,4-naphthoquinone derivatives and QSAR study. EXCLI J, 16, 714-726. doi:10.17179/excli2017-309 Phakeovilay, C., Disadee, W., Sahakitpichan, P., Sitthimonchai, S., Kittakoop, P., Ruchirawat, S., &...

Name: Mister Nirat Pholphana Office address: Research Laboratory of Chemical Carcinogenesis, Chulabhorn Research Institute, 54 Kamphaeng Phet 6 Talat Bang Khen, Lak Si, Bangkok 10210, Thailand Tel: +66 2553 8555 (ext. 3131) Fax:+662-553-8572 E-mail:[email protected] Positions:Research Scientist, Research Laboratory of Chemical Carcinogenesis, Chulabhorn Research Institute Education and Degrees B.Sc. (Medical Technology), Chulalongkorn University, 1991 M.Sc. (Microbiology), Mahidol University, 1995

Name: Dr. Pattama Singhirunnusorn Office address: Research Laboratory of Chemical Carcinogenesis, Chulabhorn Research Institute, 54 Kamphaeng Phet 6 Talat Bang Khen, Lak Si, Bangkok 10210, Thailand Tel: +662–5538555 ext. 3135 (office), +6687–5915431 (mobile) Fax:+662–5538572 Positions:Research Scientist, Research Laboratory of Chemical Carcinogenesis, Chulabhorn Research Institute E–mail:[email protected] Website:– Research Interests Signal transduction Cell and Molecular Biology Education and Degrees: B.Sc. (Chemistry), Suan Dusit Rajabhat University, Bangkok, Thailand, 1994–1997 M.Sc. (Pharmaceutical Chemistry), Mahidol University, Bangkok, Thailand, 1999–2002 Ph.D. (Pharmaceutical Science), University of Toyama, Toyama, Japan, 2002–2005 Academic Positions Researcher, Department of Pharmacognosy, Faculty of pharmacy, Mahidol University, Thailand, 1998 Postdoctoral Fellow, Division of Pathogenic Biochemistry, Department of Bioscience, Institute of Natural Medicine, University of Toyama, Japan, 2006–2008 Researcher, Chemical Carcinogenesis laboratory, Chulabhorn Research Institute, 2008–present Academic Activities Local Activities Project proposal committee (2015–2016), Thesis defend committee (2016–2017), Anti–Aging and Regenerative Medicine Faculty, Dhurakij Pundit University Fellowships Postdoctoral Fellow, Division of Pathogenic Biochemistry, Department of Bioscience, Institute of Natural Medicine, University of Toyama, Japan, 2006–2008 International Awards & Honours Toyama New Industry Organization Grants, Japan, 2006–2007 Monbukagakusho Scholarship, Japan, 2002–2005 Local Awards & Honours Poster award at The 10th National Cancer Conference, Bangkok, Thailand, 2009 ORCID iD: https://orcid.org/0000–0002–8085–5417 Publications A. Research Articles in International Journals Singhirunnusorn, P., Moolmuang, B., Lirdprapamongkol, K., & Ruchirawat, M. (2018). Arsenite exposure potentiates apoptosis–inducing effects of tumor necrosis...

Name: Dr. M.L. Siritida Rabibhadana Office address: Research Laboratory of Chemical Carcinogenesis,Chulabhorn Research Institute, 54 Kamphaeng Phet 6 Talat Bang Khen, Lak Si, Bangkok 10210, Thailand Tel: +662-5538555 ext 3130 Position: Research Scientist E-mail:[email protected] Research Interests:Cell and Molecular Biology Education and Degrees: B. Sc.(Biotechnology)Mahidol University, 1983-1987 M. Sc. (Microbiology)Mahidol University, 1987-1991 Ph. D. (Microbiology)Mahidol University, 1991-1996 Awards Award of Highest Grade Point Average from Dr. Tab Foundation, 1987 Scholarship from the National Center of Genetic Engineering and Biotechnology, 1990 Scholarship from the National Science and Technology Agency, 1991-1996 Work Activities August 1989-October 1989 Molecular Biology Training at University of Maryland, Baltimore County, USA October 1989-April 1990 Molecular Training at Oregon State University, USA September 1998-March 1999 Molecular Training at International Agency for Research on Cancer (IARC), Lyon, France June 28, 2003-July 26, 2003 Microarray Technology Training at Massachusetts Institute of Technology (MIT), Cambridge, MA, USA February 23, 2004-March 31, 2004 Microarray Technology Training at Oregon Health Science Technology (OHSU), Portland, OR, USA November 1, 2004 -January 27, 2005 ELISA Technique Training at National Cancer Institute (NCI), Bethesda, MD, USA Publications Schaffer, A. A., Dominguez, D. A., Chapman, L. M., Gertz, E. M., Budhu, A., Forgues, M., . . . Wang, X. W. (2021). Integration of adeno-associated virus (AAV) into the genomes of most Thai and Mongolian liver cancer patients does...

Name: Dr. Jittiporn Chaisaingmongkol Office address: Research Laboratory of Chemical Carcinogenesis, Chulabhorn Research Institute, 54 Kamphaeng Phet 6 Talat Bang Khen, Lak Si, Bangkok 10210, Thailand Tel: +662-5538555 ext. 3136 (office) Fax:+662-5538572 Position: Research Scientist I, Research Laboratory of Chemical Carcinogenesis, Chulabhorn Research Institute E-mail:[email protected] Research Interests Cancer biology and molecular biology (Epigenetics and DNA repair) Integrated and functional genomics of human liver cancer (Hepatocellular carcinoma and cholangiocarcinoma) Education and Degrees: 2000 – 2006 Diplom Degree (Bachelor and Master degree) Bayerischen Julius-Maximilians-Universitaet Wuerzburg, Wuerzburg, GERMANY 2006 – 2011 Doctor of Science (DR. RER. NAT.) Ruperto-Carola University of Heidelberg, Heidelberg, GERMANY Fellowships 2011 Postdoctoral Fellow at the German Cancer Research Center, Heidelberg, GERMANY 2013-2016 Visiting fellow at Laboratory of Human Carcinogenesis, National Cancer Institute, National Institute of Health, Bethesda, MD, USA ORCID iD: https://orcid.org/0000-0001-8019-5562 Publications Ma L, Heinrich S, Wang L, Keggenhoff FL, Khatib S, Forgues M, et al. Multiregional single-cell dissection of tumor and immune cells reveals stable lock-and-key features in liver cancer. Nat Commun. 2022;13(1):7533. Khatib SA, Ma L, Dang H, Forgues M, Chung JY, Ylaya K, et al. Single-cell biology uncovers apoptotic cell death and its spatial organization as a potential modifier of tumor diversity in HCC. Hepatology. 2022;76(3):599-611. Schaffer AA, Dominguez DA, Chapman LM, Gertz EM, Budhu A, Forgues M, et al. Integration of adeno-associated virus (AAV)...