Dr. Phanthakarn Tit-oon

Name : Dr. Phanthakarn Tit-oon, PhD

Office address: Translational Research Unit, Chulabhorn Research Institute,
54 Kamphaeng Phet 6 Talat Bang Khen, Lak Si, Bangkok 10210, Thailand
Tel: +662-554-1900 ext. 2617 (office)
E-mail: [email protected]
Position: Research Scientist, Translational Research Unit, Chulabhorn Research Institute

Research Interests:
1. Biopharmaceuticals
2. Research and development
3. Cell-based and ligand binding assays

Education and Degrees

• PhD (Applied Biological Sciences: Environmental Health), Chulabhorn Graduate Institute, Bangkok, Thailand, 2014.
• MSc (Biochemistry), Mahidol University, Bangkok, Thailand, 2008.
• BSc (Medical Technology), Chiang Mai University, Chiang Mai, Thailand, 2004.

Academic and Research Positions

• Research Scientist (2018-Present), Translational Research Unit, Chulabhorn Research Institute, Bangkok, Thailand.
• Postdoctoral Associate (2017-2018), David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Massachusetts, United States.
• Researcher (2015-2016), Siriraj Metabolomics and Phenomics Center, Faculty of Medicine, Siriraj Hospital, Mahidol University, Thailand.
• Lecturer (2008-2009), Department of Clinical Chemistry, Faculty of Medical Technology, Huachiew Chalermprakiet University, Samutprakan, Thailand.

Professional Societies

• Member: Protein Society of Thailand, 2022-present.
• Member: The Association of Medical Technologist of Thailand, 2017-present.

International Awards & Honours

• The best poster presentation award (honorable mention) at the 17th International Symposium of the Protein Society of Thailand (PST). Chiang Mai, Thailand. November 2022.
• The travel award for young investigators at the 12th Human Proteome Organization (HUPO) World Congress. Yokohama, Japan. September 2013.
• The outstanding abstract award at the 7th International Symposium of the Protein Society of Thailand (PST). Bangkok, Thailand. August 2012.
• The young scientist travel award at the 6th Asia Oceania Human Proteome Organization (AOHUPO) Congress. Beijing, China. May 2012.

Local Awards & Honours

• The outstanding thesis award from Faculty of Graduate Studies, Mahidol University. Bangkok, Thailand. July 2009.
• The very good poster presentation award from the 12th National Graduate Research Conference, Khon Kaen, Thailand. February 2009.

Publications
1. Tit-oon P, Wonglangka A, Boonkanta K, Ruchirawat M, Fuangthong M, Khongmanee A, and Sasisekharan R. Intact mass analysis reveals the novel O-linked glycosylation on the stalk region of PD-1 protein. (manuscript under review).

2. Iwasaki YW, Tharakaraman K, Subramanian V, Khongmanee A, Hatas A, Fleischer E, Rurak TT, Ngok- ngam P, Tit-oon P, Ruchirawat M, Satayavivad J, Fuangthong M, and Sasisekharan R. Generation of bispecific antibodies by structure guided redesign of IgG constant regions. (accepted manuscript).

3. Susantad T, Fuangthong M, Tharakaraman K, Tit-oon P, Ruchirawat M, and Sasisekharan R. Modified recombinant human erythropoietin with potentially reduced immunogenicity. Sci Rep. 2021;11(1):1491.

4. Tit-oon P, Tharakaraman K, Artpradit C, Godavarthi A, Sungkeeree P, Sasisekharan V, et al. Prediction of the binding interface between monoclonal antibody m102.4 and Nipah attachment glycoprotein using structure-guided alanine scanning and computational docking. Sci Rep. 2020;10(1):18256.

5. Akarasereenont P, Pratumvinit B, Sirivatanauksorn V, Avirutnan P, Masaratana P, Tovikkai C, Kongmanas K, Tit-oon P, Roytrakul S, Srisawat C, and Sirivatanauksorn Y. Siriraj Metabolomics and Phenomics Center. Siriraj Med J. 2016. Mar;68(2):125-126

6. Tit-oon P, Chokchaichamnankit D, Khongmanee A, Sawangareetrakul P, Svasti J, and Srisomsap C. Comparative secretome analysis of cholangiocarcinoma cell line in three-dimensional culture. Int J Oncol. 2014 Nov;45(5):2108-16.

7. Khongmanee A, Lirdprapamongkol K, Tit-oon P, Chokchaichamnankit D, Svasti J, and Srisomsap C. Proteomic analysis reveals important role of 14-3-3σ in anoikis resistance of cholangiocarcinoma cells. Proteomics. 2013 Nov;13(21):3157-66.

8. Tit-oon P. and Chotpadiwetkul R. The inhibition of metal-catalysed BSA oxidation by dietary plants. J.Med Assoc.Thai. 2004 Dec;32(3):787-795.

Conference presentations
1. Tit-oon P, Khongmanee A, Tianpanich K, Piwkam A, Wajeesaksit S, Ruchirawat M, and Fuangthong M. “Enhanced antibody production by lowering culture temperature of suspension HEK293 cells”. The 17th International Symposium of the Protein Society of Thailand (PST). November 9 – 11, 2022, Khum Kham International Convention Centre, Chiang Mai, Thailand. [Poster presentation]

2. Tit-oon P, Boonkanta K, Wonglangka A, Fuangthong M, Ruchirawat M, and Khongmanee A. TMT “Quantitative multiplexed proteomics of anoikis induced HER2-positive breast cancer cell line—BT- 474”. The 17th International Symposium of the Protein Society of Thailand (PST). November 9 – 11, 2022, Khum Kham International Convention Centre, Chiang Mai, Thailand. [Poster presentation]

3. Tit-oon P, Boonkanta K, Wonglangka A, Fuangthong M, Ruchirawat M, and Khongmanee A. “Quantitative multiplexed proteomics identifies a paradoxical role protein upregulation in breast cancer spheroid”. Proceedings of the Annual Conference of the Center of Excellence on Environmental Health and Toxicology; October 8 2022; Chulabhorn Research Institute, Bangkok, Thailand. [Poster presentation]

4. Boonkanta K, Tit-oon P, Sungkeeree P, Ngok-ngam P, Khongmanee A, Fuangthong F, Ruchirawat M, Mongkosuk S. “Development of biological reagent for immunogenicity assay of monoclonal antibody drug”. Proceedings of the Annual Conference of the Center of Excellence on Environmental Health and Toxicology. January 30, 2021, Chulabhorn Research Institute, Bangkok. p.49-53. [Poster presentation and Proceeding]

5. Wonglangka A, Tit-oon P, Ngok-ngam P, Khongmanee A, Sungkeeree P, Artpradit C, Fuangthong M, Ruchirawat M, Mongkolsuk S. “Development of in vitro immunogenicity assay for monoclonal antibody drug”. Proceedings of the Annual Conference of the Center of Excellence on Environmental Health and Toxicology. January 30, 2021; Chulabhorn Research Institute, Bangkok. p. 54-58. [Poster presentation and Proceeding]

6. Tit-oon P, Boonkanta K, Wonglangka A, Fuangthong M, Ruchirawat M, Khongmanee A. “Identification of novel O-glycans modification on key protein in immune checkpoint pathway using intact mass analysis” The 15th International Symposium of the Protein Society of Thailand (PST). November 4 – 6, 2020, Chulabhorn Research Institute Convention Center, Bangkok, Thailand. [Poster presentation]

7. Akarasereenont P, Pratumvinit B, Sirivatanauksorn V, Avirutnan P, Masaratana P, Tovikkai C, Kongmanas K, Tit-oon P, Roytrakul S, Srisawat C, Sirivatanauksorn Y. “Siriraj Metabolomics and  Phenomics Center”. The Opening Ceremony of Siriraj Metabolomics and Phenomics Center and International Conference of Metabolomics. June 9, 2016, Siriraj Hospital, Bangkok, Thailand. [Poster presentation].

8. Tit-oon P, Sawangareetrakul P, Chokchaichamnankit D, Svasti J, and Srisomsap C. “Secretome analysis of three-dimensional in vitro model cholangiocarcinoma” The 12th Human Proteome Organization (HUPO) World Congress. September 14 -18, 2013, Pacifico Yokohama, Yokohama, Japan. [Selected oral presentation].

9. Tit-oon P, Diskul Na Ayudthaya P, Chiablaem K, Chokchaichamnankit D, Svasti J, and Srisomsap C. “Culture of cholangiocarcinoma cells in 3-dimensional architecture alters protein expression profiles” The 7th Princess Chulabhorn International Science Congress (PC VII). November 29 – December 3, 2012, Shangri-La Hotel, Bangkok, Thailand. [Poster presentation].

10. Tit-oon P, Diskul Na Ayudthaya P, Chiablaem K, Chokchaichamnankit D, Svasti J, and Srisomsap C. “Comparison of 3D and 2D cholangiocarcinoma models reveals different biological characteristics” The 7th International Symposium of the Protein Society of Thailand (PST). August 29 – 31, 2012, Chulabhorn Research Institute Convention Center, Bangkok, Thailand. [Selected oral presentation].

11. Khongmanee A, Lirdprapamongkol K, Tit-oon P, Chokchaichamnankit D, Svasti J, Srisomsap C. “Proteomic analysis of anoikis resistant cholangiocarcinoma cell line” The 6th Asia Oceania Human Proteome Organization (AOHUPO) Congress. May 5 – 7, 2012, China National Convention Center, Beijing, China. [Poster presentation].

12. Tit-oon P, Diskul Na Ayudthaya P, Chiablaem K, Chokchaichamnankit D, Svasti J, and Srisomsap C. “Comparison of 3D and 2D cholangiocarcinoma models reveals different biological characteristics” The 6th Asia Oceania Human Proteome Organization (AOHUPO) Congress. May 5 – 7, 2012, China National Convention Center, Beijing, China. [Poster presentation].

13. Tit-oon P, Panyawirunroj T, Chuangsuwanich A, Limwongse C, and Srisawat C. “Inhibition of collagen production in keloid fibroblasts using RNA interference” The 12th National Graduate Research Conference. February 12-13, 2009, Khon Kaen University, Khon Kaen, Thailand. [Poster presentation].

14. Tit-oon P, Panyawirunroj T, Chuangsuwanich A, Limwongse C, and Srisawat C. “Inhibition of collagen production in keloid fibroblasts using collagen-targeted RNA interference” The 120th year’s anniversary of Siriraj Hospital scientific congress. March 17-21, 2008, Siriraj Hospital, Bangkok, Thailand [Selected oral presentation].

15. Chotpadiwetkul R. and Tit-oon P. “Protection against oxidative protein damage by plant extracts” The 10th ASEAN Conference in Medical Laboratory Technology. April 26-30, 2004, Lotus Pang Suan Kaew, Chiang Mai, Thailand. [Poster presentation].